Azidothymidine is effective against human multiple myeloma
dc.contributor.author | PEREIRA, JULIANA | pt_BR |
dc.contributor.author | LEVY, DEBORA | pt_BR |
dc.contributor.author | RUIZ, JORGE L.M. | pt_BR |
dc.contributor.author | BROCARDO, GRACIELA A. | pt_BR |
dc.contributor.author | FERREIRA, KLEBER A. | pt_BR |
dc.contributor.author | COSTA, RENATA O. | pt_BR |
dc.contributor.author | QUEIROZ, RODRIGO G. | pt_BR |
dc.contributor.author | MARIA, DURVANEI A. | pt_BR |
dc.contributor.author | HALLACK NETO, ABRAHÃO E. | pt_BR |
dc.contributor.author | CHAMONE, DALTON A.F. | pt_BR |
dc.contributor.author | BYDLOWSKI, SÉRGIO P. | pt_BR |
dc.coverage | Internacional | pt_BR |
dc.date.accessioned | 2014-07-31T11:35:55Z | pt_BR |
dc.date.accessioned | 2014-07-31T11:52:54Z | |
dc.date.available | 2014-07-31T11:35:55Z | pt_BR |
dc.date.available | 2014-07-31T11:52:54Z | |
dc.date.issued | 2013 | pt_BR |
dc.description.abstract | Azidothymidine (AZT) is an antiretroviral drug that affects cell proliferation, apoptosis, and the NF-κB pathway. As multiple myeloma (MM) presents with constitutive activation of NF-κB, we analyzed the effect of AZT on human MM cell lines. We evaluated the cytotoxic effect of AZT in human MM cell lines sensitive (8226/S) or resistant to doxorubicin (8226/DX5) and human T cell lymphoblast-like cells, uterine sarcoma cells, and HUVEC using MTT assay. Cytotoxicity was also evaluated in vivo in nude mice xenografted with 8226/S tumor. The effect of AZT on the expression of genes involved in cell proliferation, apoptosis, angiogenesis, and the NF-κB pathway was analyzed in the xenografts using real-time polymerase chain reaction. AZT was effective against both 8226/S and 8226/DX5 cells in a dose and time-dependent manner (p = 0.02) in vitro and promoted cell cycle arrest in S phase in these cells. The tumor volume was lower in mice treated with AZT compared to untreated mice (p = 0.0003). AZT down-regulated the pro-proliferative genes encoding AKT1, MYC, STAT1, MAPK8, MAPK9, CCL-3, Bcl-3, and cyclin D2; pro-angiogenenic genes encoding VEGF and IL8; and genes involved in cell adhesion (ICAM1 and FN1) and the NF-κB pathway. AZT up-regulated the expression of tumor suppressor gene FOXP1 and the pro-apoptotic genes encoding BID, Bcl-10, and caspase-8. Thus, we demonstrated the cytotoxic effect of AZT in human MM cell lines for the first time. Our data may provide the rationale for future clinical trials of AZT for treating MM. | |
dc.format.extent | 186-192 | pt_BR |
dc.identifier.citation | PEREIRA, JULIANA; LEVY, DEBORA; RUIZ, JORGE L.M.; BROCARDO, GRACIELA A.; FERREIRA, KLEBER A.; COSTA, RENATA O.; QUEIROZ, RODRIGO G.; MARIA, DURVANEI A.; HALLACK NETO, ABRAHÃO E.; CHAMONE, DALTON A.F.; BYDLOWSKI, SÉRGIO P. Azidothymidine is effective against human multiple myeloma: a new use for an old drug?. <b>Anti-Cancer Agents in Medicinal Chemistry</b>, v. 13, n. 1, p. 186-192, 2013. DOI: <a href="https://dx.doi.org/10.2174/187152013804487416">10.2174/187152013804487416</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/8476. | |
dc.identifier.doi | 10.2174/187152013804487416 | |
dc.identifier.fasciculo | 1 | pt_BR |
dc.identifier.issn | 1871-5206 | pt_BR |
dc.identifier.uri | http://repositorio.ipen.br/handle/123456789/8476 | pt_BR |
dc.identifier.vol | 13 | pt_BR |
dc.relation.ispartof | Anti-Cancer Agents in Medicinal Chemistry | pt_BR |
dc.rights | closedAccess | en |
dc.subject | neoplasms | pt_BR |
dc.subject | plasma cells | pt_BR |
dc.subject | hemic diseases | pt_BR |
dc.subject | mortality | pt_BR |
dc.subject | anti-infective agents | pt_BR |
dc.subject | viral diseases | pt_BR |
dc.subject | cell proliferation | pt_BR |
dc.subject | apoptosis | pt_BR |
dc.subject | angiogenesis | pt_BR |
dc.subject | toxicity | pt_BR |
dc.title | Azidothymidine is effective against human multiple myeloma | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dspace.entity.type | Publication | |
ipen.autor | DURVANEI AUGUSTO MARIA | |
ipen.autor | RODRIGO GUIMARÃES QUEIROZ | |
ipen.codigoautor | 12160 | |
ipen.codigoautor | 6931 | |
ipen.contributor.ipenauthor | DURVANEI AUGUSTO MARIA | |
ipen.contributor.ipenauthor | RODRIGO GUIMARÃES QUEIROZ | |
ipen.date.recebimento | 13-12 | pt_BR |
ipen.identifier.fi | 2.939 | pt_BR |
ipen.identifier.ipendoc | 19519 | pt_BR |
ipen.identifier.iwos | WoS | pt_BR |
ipen.identifier.ods | 3 | |
ipen.range.fi | 1.500 - 2.999 | |
ipen.subtitulo | a new use for an old drug? | |
ipen.type.genre | Artigo | |
relation.isAuthorOfPublication | 42c5c181-64cf-4d75-8e17-c2fa548d8b61 | |
relation.isAuthorOfPublication | 53d3df0d-4e12-4db7-99fa-dd742a7070fe | |
relation.isAuthorOfPublication.latestForDiscovery | 53d3df0d-4e12-4db7-99fa-dd742a7070fe | |
sigepi.autor.atividade | PEREIRA, JULIANA:-1:-1:S | pt_BR |
sigepi.autor.atividade | LEVY, DEBORA:-1:-1:N | pt_BR |
sigepi.autor.atividade | RUIZ, JORGE L.M.:-1:-1:N | pt_BR |
sigepi.autor.atividade | BROCARDO, GRACIELA A.:-1:-1:N | pt_BR |
sigepi.autor.atividade | FERREIRA, KLEBER A.:-1:-1:N | pt_BR |
sigepi.autor.atividade | COSTA, RENATA O.:-1:-1:N | pt_BR |
sigepi.autor.atividade | QUEIROZ, RODRIGO G.:6931:-1:N | pt_BR |
sigepi.autor.atividade | MARIA, DURVANEI A.:12160:-1:N | pt_BR |
sigepi.autor.atividade | HALLACK NETO, ABRAHÃO E.:-1:-1:N | pt_BR |
sigepi.autor.atividade | CHAMONE, DALTON A.F.:-1:-1:N | pt_BR |
sigepi.autor.atividade | BYDLOWSKI, SÉRGIO P.:-1:-1:N | pt_BR |
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