Analysis of the proteome and biochemistry of venom from Tityus Confluens, a scorpion that can be involved in severe envenomation cases in Brazil
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In Brazil, the annual scorpion sting cases surpass those of other neglected tropical diseases,
highlighting a significant public health issue. The severity of scorpion envenomation
relates to the venom’s rapid action, complex composition, species identification challenges,
and limited antivenom availability. This work aimed to characterize the venom of
Tityus confluens through proteomic, enzymatic, and biological analyses while also assessing
its reactivity to anti-scorpion antivenom. The electrophoretic analysis revealed seven
protein bands, with the most prominent bands at 30, 15, and 10 kDa. The C18-RP-HPLC
analysis isolated sixteen primary fractions. The proteomic analysis identified various
toxins, including potassium channel toxins, sodium channel toxins, and antimicrobial
peptides, as well as other proteins such as hypotensin and metalloproteinases. Antigenic
components were identified in the T. confluens venom, which displayed dose-dependent
but time-independent amylolytic activity. The ATPase activity significantly increased with
1–10 μg of venom. No cytotoxic effects were observed on carcinoma or non-tumoral cell
lines. The T. confluens venom features a complex protein composition rich in toxins that
target ion channels and enzymes. It exhibits active enzymatic and antigenic properties,
and displays low cytotoxicity. This is the first proteomic research on the composition
of T. confluens venom and may provide valuable insights into understanding the clinical
manifestations of scorpion stings.
Como referenciar
LIMA, LAIS C.; COVALI-PONTES, HENRIQUE R.; LEITE OHANNA G.S.; PERDOMO, RENATA T.; MORAES, LUIZ F.R.N. de; MIGLIOLO, LUDOVICO; MOYSES, MAURICIO N.; SANTOS, NATALIA G.P. dos; PIMENTA, DANIEL C.; RODRIGUES, MARIANA S.; MORAIS-ZANI, KAREN; COELHO, GUILHERME R.; LUCENA, MALSON N. Analysis of the proteome and biochemistry of venom from Tityus Confluens, a scorpion that can be involved in severe envenomation cases in Brazil. Toxins, v. 17, n. 8, p. 1-16, 2025. DOI: 10.3390/toxins17080406. Disponível em: https://repositorio.ipen.br/handle/123456789/49517. Acesso em: 24 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.