Effects of gamma radiation on the pBs-KS DNA plasmid

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dc.contributor.authorARRUDA NETO, J.D.T.pt_BR
dc.contributor.authorZAMBONI, C.B.pt_BR
dc.contributor.authorDUARTE, C.L.pt_BR
dc.contributor.authorSEMMLER, R.pt_BR
dc.contributor.authorJORGE, S.A.C.pt_BR
dc.contributor.authorARAUJO, G.W.pt_BR
dc.contributor.authorSHTEJER, K.pt_BR
dc.contributor.authorGOUVEIA, A.N.pt_BR
dc.contributor.authorDEPPMAN, A.pt_BR
dc.contributor.authorHELENE, O.A.M.pt_BR
dc.contributor.authorVANIN, V.R.pt_BR
dc.contributor.authorLIKHACHEV, V.P.pt_BR
dc.contributor.authorMANSO GUEVARA, M.V.pt_BR
dc.contributor.authorMIRANDA, A.pt_BR
dc.contributor.authorRODRIGUEZ, O.pt_BR
dc.coverageNacionalpt_BR
dc.creator.eventoREUNIAO DE TRABALHO SOBRE FISICA NUCLEAR NO BRASIL, 24.pt_BR
dc.date.accessioned2014-11-19T11:30:02Zpt_BR
dc.date.accessioned2014-11-19T14:10:24Zpt_BR
dc.date.accessioned2015-04-01T13:45:36Z
dc.date.available2014-11-19T11:30:02Zpt_BR
dc.date.available2014-11-19T14:10:24Zpt_BR
dc.date.available2015-04-01T13:45:36Z
dc.date.evento1-5 set, 2001pt_BR
dc.description.abstractDNA is considered to be the most important and critical target in a cell from the point of view of radiation damage. It is responsible for conservation and transmission of all the cell genetic information. It is constantly submitted to different kind of damages, where repair could or couldn't take place. The energy transferred by ionizing radiation to the DNA strands can induce mutation, carcinogenic process and cell death. The DNA damage involves nucleotide base alterations, single (SSB) and double (DSB) strand breaks and also chromatin rupture. The DNA strand break (mainly DSB) is the most critical damage induced by ionizing radiation. Specifically. gamma radiation is used in a large number of illness treatment including cancer diagnosis, treatment and cure. In fact, this radiation is the base of conventional radiotherapy using ®9Co and ?%7Cs sources. Beside, this radiation is also present in treatment which involves neutrons and others types of particles, such as protons, alphas and heavy ions ( e.g. Ne. Ar). The 250 MeV proton beam therapy, largely used in recent years as an effective non invasive cancer treatment. produces gamma rays as a secondary radiation which can generate radiobiological effects on healthy tissue. This kind of radiation with low linear energy transfer (LET) interacts with the tissue producing secondary electrons. which directly produce excited and ionized states into the DNA strands and inducing the formation of energetic free radicals in the aqueous solution containing the DNA molecule. The DNA strand damages produced by these interactions must be well known in order to prevent the radiobiological effects on human being The investigation of DNA radiation effects has been very intensive in the last years!'=3), and the present study is a contribution to better understand the mechanism of single (SSB) and double (DSB) strand breaks of pBs KS (+) plasmid DNA with 1 MeV gamma radiation. About 10 ml of DNA was irradiated at a concentration of 15 ng/ml in a cylindrical plastic tube (eppendorf) in the ®®Co Gamma Cell at IPEN ( Instituto de Pesquisas Energeticas e Nucleares, Sao Paulo) facility. The dose rate was 5 kGy/h, and the biological material was submitted to total doses ranging from 0 to 200 Gy. Supercoiled (FI),Circular (FII) and Linear (FIII) forms of the plasmid were separated by agarose gel electrophoresis at 10 volts overnight with 1% agarose. The results exhibit a decreasing of the FI plasmid fraction and an increasing number of the FII and FIII fractions with the dose, suggesting the presence of SSBs and DSBs in the irradiated DNA plasmid. The detectable molecule fractions of each form of plasmid were analyzed by means of a statistical treatment[%) allowing the calculation of the average number of SSB and DSB per plasmid for each interaction dose.These two quantities are shown in the figure. The curve is only to guide the eyes(preliminary data). In this analysis it was assumed that the strand break distribution obeys Poisson' laws. These calculations were compared with the corresponding experimental data. It was found that the statistical results describe satisfactory the data for gamma radiation.
dc.event.siglaRTFNB,24pt_BR
dc.format.extent61pt_BR
dc.identifier.citationARRUDA NETO, J.D.T.; ZAMBONI, C.B.; DUARTE, C.L.; SEMMLER, R.; JORGE, S.A.C.; ARAUJO, G.W.; SHTEJER, K.; GOUVEIA, A.N.; DEPPMAN, A.; HELENE, O.A.M.; VANIN, V.R.; LIKHACHEV, V.P.; MANSO GUEVARA, M.V.; MIRANDA, A.; RODRIGUEZ, O. Effects of gamma radiation on the pBs-KS DNA plasmid. In: REUNIAO DE TRABALHO SOBRE FISICA NUCLEAR NO BRASIL, 24., 1-5 set, 2001, Aguas de Lindoia, SP. <b>Resumos...</b> p. 61. Disponível em: http://repositorio.ipen.br/handle/123456789/20772.
dc.identifier.orcidhttps://orcid.org/0000-0001-7733-0153
dc.identifier.orcidhttps://orcid.org/0000-0001-5739-7502
dc.identifier.orcidhttps://orcid.org/0000-0001-7766-2692
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/20772pt_BR
dc.local.eventoAguas de Lindoia, SPpt_BR
dc.rightsopenAccess
dc.subjectbiological radiation effectspt_BR
dc.subjectgamma radiationpt_BR
dc.subjectcobalt 60pt_BR
dc.subjectstrand breakspt_BR
dc.subjectdnapt_BR
dc.subjectplasmidspt_BR
dc.subjectradiation dosespt_BR
dc.subjectelectrophoresispt_BR
dc.subjectradiotherapypt_BR
dc.titleEffects of gamma radiation on the pBs-KS DNA plasmidpt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorRENATO SEMMLER
ipen.autorCELINA LOPES DUARTE
ipen.autorCIBELE BUGNO ZAMBONI
ipen.codigoautor833
ipen.codigoautor143
ipen.codigoautor267
ipen.contributor.ipenauthorRENATO SEMMLER
ipen.contributor.ipenauthorCELINA LOPES DUARTE
ipen.contributor.ipenauthorCIBELE BUGNO ZAMBONI
ipen.date.recebimento02-04pt_BR
ipen.event.datapadronizada2001pt_BR
ipen.identifier.ipendoc08022pt_BR
ipen.notas.internasResumospt_BR
ipen.type.genreResumo
relation.isAuthorOfPublicationf95a9324-04eb-409b-86b2-97eaa10134f2
relation.isAuthorOfPublicationca559095-3ff4-482d-9d4b-c9662441e5ba
relation.isAuthorOfPublication563d95e5-227e-456d-a1d7-4bb051ae51ba
relation.isAuthorOfPublication.latestForDiscovery563d95e5-227e-456d-a1d7-4bb051ae51ba
sigepi.autor.atividadeARRUDA NETO, J.D.T.:6496:-1:Spt_BR
sigepi.autor.atividadeZAMBONI, C.B.:267:6:Npt_BR
sigepi.autor.atividadeDUARTE, C.L.:143:2:Npt_BR
sigepi.autor.atividadeSEMMLER, R.:833:6:Npt_BR
sigepi.autor.atividadeJORGE, S.A.C.:-1:-1:Npt_BR
sigepi.autor.atividadeARAUJO, G.W.:-1:-1:Npt_BR
sigepi.autor.atividadeSHTEJER, K.:8821:-1:Npt_BR
sigepi.autor.atividadeGOUVEIA, A.N.:-1:-1:Npt_BR
sigepi.autor.atividadeDEPPMAN, A.:787:-1:Npt_BR
sigepi.autor.atividadeHELENE, O.A.M.:3718:-1:Npt_BR
sigepi.autor.atividadeVANIN, V.R.:11235:-1:Npt_BR
sigepi.autor.atividadeLIKHACHEV, V.P.:-1:-1:Npt_BR
sigepi.autor.atividadeMANSO GUEVARA, M.V.:3609:-1:Npt_BR
sigepi.autor.atividadeMIRANDA, A.:681:-1:Npt_BR
sigepi.autor.atividadeRODRIGUEZ, O.:-1:-1:Npt_BR
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