Microfluidic caging lipase in hyperbranched polyglycerol microcapsules for extracorporeal treatment of enzyme pancreatic insufficiency

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dc.contributor.authorALVES, ANDRESSA A.pt_BR
dc.contributor.authorQUEIROZ, ALFREDO A.A.E. dept_BR
dc.contributor.authorSOARES, CARLOS R.J.pt_BR
dc.contributor.authorQUEIROZ, ALVARO A.A. dept_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2021-12-29T13:25:52Z
dc.date.available2021-12-29T13:25:52Z
dc.date.issued2021pt_BR
dc.description.abstractLipase cartridges are currently the mainstay of treatment to improve fat absorption related to pancreatic insufficiency (PI) in patients receiving enteral nutrition feedings. Enzyme immobilization is an essential prerequisite for designing lipase cartridges systems for efficient enzymatic fat hydrolysis. A microfluidic approach has been adopted to produce lipase (LIP) caged in hyperbranched polyglycerol microcapsules (HPGly). The resulting HPGly-LIP microcapsules are spherical and had an average diameter of 29 µm with monomodal size distribution. The optimum conditions determined by artificial neural networks were HPGly concentration of 10 wt.%, LIP loading of 20% (wt) and total flow rate in microfluidic cell of 1.0 mL/h. Under these conditions, the maximum capacity of the LIP that can be microencapsulated is around 85% with respect to the HPGly concentration of 10 wt.% and total flow rate in microfluidic cell of 1.0 mL/h. This resultant HPGly-LIP exhibited Michaelis–Menten coefficients of 1.138,14 mM (Km) and 0.49 U/mg (Vmax) showing higher activity compared to free LIP. Finally, the robust HPGly-LIP microcapsules showed excellent recyclability. The in vitro Analysis of the HPGly-LIP cytotoxicity showed that microcapsules had no cytotoxic effect to L929 fibroblasts cells and behaved very similar to the negative control. These features will be useful for the facile construction of biocatalytic systems with high efficiency, excellent recyclability and adequate biocompatibility for treatment of patients with PI receiving enteral nutrition feedings.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipIDCNPq: 307609/2018-9pt_BR
dc.format.extent2349-2368pt_BR
dc.identifier.citationALVES, ANDRESSA A.; QUEIROZ, ALFREDO A.A.E. de; SOARES, CARLOS R.J.; QUEIROZ, ALVARO A.A. de. Microfluidic caging lipase in hyperbranched polyglycerol microcapsules for extracorporeal treatment of enzyme pancreatic insufficiency. <b>Journal of Biomaterials Science, Polymer Edition</b>, v. 32, n. 18, p. 2349-2368, 2021. DOI: <a href="https://dx.doi.org/10.1080/09205063.2021.1971820">10.1080/09205063.2021.1971820</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/32551.
dc.identifier.doi10.1080/09205063.2021.1971820pt_BR
dc.identifier.fasciculo18pt_BR
dc.identifier.issn0920-5063pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7982-1789
dc.identifier.percentilfi50.01pt_BR
dc.identifier.percentilfiCiteScore66.00pt_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/32551
dc.identifier.vol32pt_BR
dc.relation.ispartofJournal of Biomaterials Science, Polymer Editionpt_BR
dc.rightsclosedAccesspt_BR
dc.subjectpancreas
dc.subjectinflammation
dc.subjectlipases
dc.subjectglycerol
dc.subjecttoxicity
dc.titleMicrofluidic caging lipase in hyperbranched polyglycerol microcapsules for extracorporeal treatment of enzyme pancreatic insufficiencypt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorÁLVARO ANTONIO ALENCAR DE QUEIROZ
ipen.autorCARLOS ROBERTO JORGE SOARES
ipen.codigoautor7319
ipen.codigoautor509
ipen.contributor.ipenauthorÁLVARO ANTONIO ALENCAR DE QUEIROZ
ipen.contributor.ipenauthorCARLOS ROBERTO JORGE SOARES
ipen.date.recebimento21-12
ipen.identifier.fi3.682pt_BR
ipen.identifier.fiCiteScore5.9pt_BR
ipen.identifier.ipendoc28344pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi3.000 - 4.499
ipen.range.percentilfi50.00 - 74.99
ipen.type.genreArtigo
relation.isAuthorOfPublicatione7d5bfcd-02bd-4e2a-8b3a-b4a56eb4cdfa
relation.isAuthorOfPublicationd6719ab2-f2e9-4d7c-9cea-a6316fc14c9e
relation.isAuthorOfPublication.latestForDiscoveryd6719ab2-f2e9-4d7c-9cea-a6316fc14c9e
sigepi.autor.atividadeQUEIROZ, ALVARO A.A. de:7319:-1:Npt_BR
sigepi.autor.atividadeSOARES, CARLOS R.J.:509:810:Npt_BR
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