Flow cytometry based micronucleus assay for evaluation of genotoxic potential of 2-ACBs in hepatic cells HepG2

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dc.contributor.authorBARBEZAN, ANGELICA B.pt_BR
dc.contributor.authorSANTOS, CARLA J.B.pt_BR
dc.contributor.authorCARVALHO, LUMA R.pt_BR
dc.contributor.authorVIEIRA, DANIEL P.pt_BR
dc.contributor.authorSANTELLI, GLAUCIA M.M.pt_BR
dc.contributor.authorVILLAVICENCIO, ANNA L.C.H.pt_BR
dc.coverageNacionalpt_BR
dc.date.accessioned2019-07-31T13:19:24Z
dc.date.available2019-07-31T13:19:24Z
dc.date.issued2019pt_BR
dc.description.abstractFood irradiation is approved for use in more than 60 countries for applications and purposes in a wide variety of foods, being an effective and safe method for preservation and long-term storage. 2- Alkylcyclobutanones (2-ACBs) are the only known radiolytic products generated from foods that contain fatty acids (Triglycerides) when irradiated. The acids analyzed in this study are palmitic and stearic, which when irradiated form 2-Dodecylcyclobutanones (2-dDCB) and 2-Tetradecylcyclobutanone (2-tDCB). Part of the 2-ACBs ingested is excreted through feces and part is deposited in adipose tissues. In vitro studies so far have been only in colon cells. The work used a human hepatoma cell line (HepG2) since the accumulation of fat in this organ is quite common. Micronucleus test was selected to evaluate possible genotoxic effects of 2-dDCB and 2-tDCB compounds when exposed to high concentrations (447, 1422 and 2235 μM) for 4 and 24 hours. Tests were performed in quadriplicates using flow cytometric analysis. None detectable genotoxic damage was observed after 4 hours of exposure to the compounds, and cytotoxic effects were only significant at the highest concentration (2235 μM) of 2-dDCB. After 24 hours of exposure, slight genotoxic damage was observed at all concentrations evaluated, and cytotoxic effects were only present when exposed to compound 2-tDCB. Although there is a genotoxic and cytotoxic effect in some of the situations tested, the two compounds predominantly induced proliferation reduction effects of this hepatic tumor cell line.pt_BR
dc.format.extent1-24pt_BR
dc.identifier.citationBARBEZAN, ANGELICA B.; SANTOS, CARLA J.B.; CARVALHO, LUMA R.; VIEIRA, DANIEL P.; SANTELLI, GLAUCIA M.M.; VILLAVICENCIO, ANNA L.C.H. Flow cytometry based micronucleus assay for evaluation of genotoxic potential of 2-ACBs in hepatic cells HepG2. <b>Brazilian Journal of Radiation Sciences</b>, v. 7, n. 2A, p. 1-24, 2019. DOI: <a href="https://dx.doi.org/10.15392/bjrs.v7i2A.684">10.15392/bjrs.v7i2A.684</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/29987.
dc.identifier.doi10.15392/bjrs.v7i2A.684pt_BR
dc.identifier.fasciculo2Apt_BR
dc.identifier.issn2319-0612pt_BR
dc.identifier.orcid0000-0001-6199-7877pt_BR
dc.identifier.orcid0000-0002-0007-534Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-6199-7877
dc.identifier.orcidhttps://orcid.org/0000-0002-0007-534X
dc.identifier.percentilfiSem Percentilpt_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/29987
dc.identifier.vol7pt_BR
dc.relation.ispartofBrazilian Journal of Radiation Sciencespt_BR
dc.rightsopenAccesspt_BR
dc.sourceThe Meeting on Nuclear Applications (ENAN), 13th, 22-27 de outubro, 2017, Belo Horizonte, MGpt_BR
dc.subjectfood processing
dc.subjectirradiation
dc.subjecthexadecanoic acid
dc.subjectcell flow systems
dc.subjectdodecyl radicals
dc.subjecthepatomas
dc.subjectin vitro
dc.subjectoctadecanoic acid
dc.subjectradiation effects
dc.subjecttoxicity
dc.subjectcarcinomas
dc.titleFlow cytometry based micronucleus assay for evaluation of genotoxic potential of 2-ACBs in hepatic cells HepG2pt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorCARLA JULIANA BALBINO DOS SANTOS
ipen.autorANNA LUCIA CASANAS HAASIS VILLAVICENCIO
ipen.autorDANIEL PEREZ VIEIRA
ipen.autorLUMA RAMIREZ DE CARVALHO
ipen.autorANGELICA BUENO BARBEZAN
ipen.codigoautor14679
ipen.codigoautor1372
ipen.codigoautor3158
ipen.codigoautor12133
ipen.codigoautor6417
ipen.contributor.ipenauthorCARLA JULIANA BALBINO DOS SANTOS
ipen.contributor.ipenauthorANNA LUCIA CASANAS HAASIS VILLAVICENCIO
ipen.contributor.ipenauthorDANIEL PEREZ VIEIRA
ipen.contributor.ipenauthorLUMA RAMIREZ DE CARVALHO
ipen.contributor.ipenauthorANGELICA BUENO BARBEZAN
ipen.date.recebimento19-07
ipen.identifier.fiSem F.I.pt_BR
ipen.identifier.ipendoc25774pt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublication60015783-d6e0-44c6-addb-9f55d1565d2d
relation.isAuthorOfPublication8ce58b2f-23cf-45b5-a8c4-0fb067965096
relation.isAuthorOfPublicationc2352608-be9c-4a73-be8c-571f10bb53d2
relation.isAuthorOfPublication8e39b9fc-86ca-4b06-bf2a-cb8f163165d8
relation.isAuthorOfPublication27082032-b0c8-462c-8d6c-767873119adf
relation.isAuthorOfPublication.latestForDiscovery27082032-b0c8-462c-8d6c-767873119adf
sigepi.autor.atividadeVILLAVICENCIO, ANNA L.C.H.:1372:210:Npt_BR
sigepi.autor.atividadeVIEIRA, DANIEL P.:3158:810:Npt_BR
sigepi.autor.atividadeCARVALHO, LUMA R.:12133:810:Npt_BR
sigepi.autor.atividadeSANTOS, CARLA J.B.:14679:210:Npt_BR
sigepi.autor.atividadeBARBEZAN, ANGELICA B.:6417:110:Spt_BR
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