Using fourier transform infrared spectroscopy to evaluate biological effects induced by photodynamic therapy

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dc.contributor.authorLIMA, CASSIO A.
dc.contributor.authorGOULART, VIVIANE P.
dc.contributor.authorCORREA, LUCIANA
dc.contributor.authorZEZELL, DENISE M.
dc.coverageInternacionalpt_BR
dc.date.accessioned2016-07-28T11:43:53Z
dc.date.available2016-07-28T11:43:53Z
dc.date.issued2016pt_BR
dc.description.abstractBackground and Objective: Vibrational spectroscopic methods associated with multivariate statistical techniques have been succeeded in discriminating skin lesions from normal tissues. However, there is no study exploring the potential of these techniques to assess the alterations promoted by photodynamic effect in tissue. The present study aims to demonstrate the ability of Fourier Transform Infrared (FTIR) spectroscopy on Attenuated total reflection (ATR) sampling mode associated with principal component-linear discriminant analysis (PC-LDA) to evaluate the biochemical changes caused by photodynamic therapy (PDT) in skin neoplastic tissue. Materials and Methods: Cutaneous neoplastic lesions, precursors of squamous cell carcinoma (SCC), were chemically induced in Swiss mice and submitted to a single session of 5-aminolevulinic acid (ALA)-mediated PDT. Tissue sections with 5mm thickness were obtained from formalin-fixed paraffin-embedded (FFPE) and processed prior to the histopathological analysis and spectroscopic measurements. Spectra were collected in mid-infrared region using a FTIR spectrometer on ATR sampling mode. Principal Component-Linear Discriminant Analysis (PC-LDA) was applied on preprocessed second derivatives spectra. Biochemical changes were assessed using PCA-loadings and accuracy of classification was obtained from PC-LDA . Results: Sub-bands of Amide I (1,624 and 1,650 cm1 ) and Amide II (1,517 cm1 ) indicated a protein overexpression in non-treated and post-PDT neoplastic tissue compared with healthy skin, as well as a decrease in collagen fibers (1,204, 1,236, 1,282, and 1,338 cm1 ) and glycogen (1,028, 1,082, and 1,151 cm1 ) content. Photosensitized neoplastic tissue revealed shifted peak position and decreased b-sheet secondary structure of proteins (1,624 cm1 ) amount in comparison to non-treated neoplastic lesions. PC-LDA score plots discriminated non-treated neoplastic skin spectra from post-PDT cutaneous lesions with accuracy of 92.8%, whereas non-treated neoplastic skin was discriminated from healthy tissue with 93.5% accuracy and post-PDT cutaneous lesions was discriminated from healthy tissue with 89.7% accuracy. Conclusion: PC-LDA was able to discriminate ATR-FTIR spectra of non-treated and post-PDT neoplastic lesions, as well as from healthy skin. Thus, the method can be used for early diagnosis of premalignant skin lesions, as well as to evaluate the response to photodynamic treatment.
dc.format.extent538-545pt_BR
dc.identifier.citationLIMA, CASSIO A.; GOULART, VIVIANE P.; CORREA, LUCIANA; ZEZELL, DENISE M. Using fourier transform infrared spectroscopy to evaluate biological effects induced by photodynamic therapy. <b>Lasers in Surgery and Medicine</b>, v. 48, p. 538-545, 2016. DOI: <a href="https://dx.doi.org/10.1002/lsm.22473">10.1002/lsm.22473</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/26486.
dc.identifier.doi10.1002/lsm.22473
dc.identifier.issn0196-8092pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7404-9606
dc.identifier.percentilfi66.31
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/26486
dc.identifier.vol48pt_BR
dc.relation.ispartofLasers in Surgery and Medicinept_BR
dc.rightsclosedAccesspt_BR
dc.subjectaminolevulinic acid
dc.subjectcarcinomas
dc.subjectfourier transform spectrometers
dc.subjectinfrared spectrometers
dc.subjectbiological effects
dc.subjecttherapy
dc.subjectin vivo
dc.subjectanimals
dc.subjectmice
dc.subjectlasers
dc.titleUsing fourier transform infrared spectroscopy to evaluate biological effects induced by photodynamic therapypt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorDENISE MARIA ZEZELL
ipen.autorVIVIANE PEREIRA GOULART
ipen.autorCASSIO APARECIDO LIMA
ipen.codigoautor693
ipen.codigoautor6603
ipen.codigoautor11396
ipen.contributor.ipenauthorDENISE MARIA ZEZELL
ipen.contributor.ipenauthorVIVIANE PEREIRA GOULART
ipen.contributor.ipenauthorCASSIO APARECIDO LIMA
ipen.date.recebimento16-07pt_BR
ipen.identifier.fi2.312pt_BR
ipen.identifier.ipendoc22385pt_BR
ipen.identifier.ods3
ipen.range.fi1.500 - 2.999
ipen.range.percentilfi50.00 - 74.99
ipen.type.genreArtigo
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relation.isAuthorOfPublicatione9e0d5d6-99ac-40b5-a57c-5ec82d671ec9
relation.isAuthorOfPublication72af3b0f-7d99-44eb-a234-9027c26dc589
relation.isAuthorOfPublication.latestForDiscovery72af3b0f-7d99-44eb-a234-9027c26dc589
sigepi.autor.atividadeLIMA, CASSIO A.:11396:-1:S
sigepi.autor.atividadeGOULART, VIVIANE P.:6603:-1:N
sigepi.autor.atividadeZEZELL, DENISE M.:693:920:N
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