Evaluation of structural changes of benzocaine-loaded, optimized nanostructured lipid carriers using SANS and Raman imaging approaches
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2021
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CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th
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Local anesthetics are substances that reversibly block the nerve-impulse conduction, alleviating pain without loss of
consciousness. Benzocaine, a poorly soluble local anesthetic, is an ester of para-aminobenzoic acid. Several
strategies of formulations can be used to improve bioavailability and decrease adverse effects of benzocaine. In this
study nanostructured lipid carriers (NLC) were employed. These lipid-based drug delivery carriers have a lipid core
composed of a blend of solid and liquid lipids, and a shelf of non-ionic surfactant. The main aim of this work was to
optimize benzocaine-loaded NLC and to investigate structural changes in these nanoparticles, under different
temperatures. The ratio of excipients (cetyl palmitate, Capmul® PG-8 NF and Pluronic®F68) and benzocaine in the
NLC was optimized using a 2 3 factorial design with respect to the following parameters: particle size, polydispersity
index (PDI) and zeta potentials. The interactions between the factors were found relevant to determine particle size
and PDI. Using desirability function, the best formulation conditions were found. Structural changes in optimized NLC
were observed with Small-Angle Neutron Scattering (SANS) and Raman imaging, in samples at 27, 37 and 40º C.
SANS pointed the formation of lamellar structures inside the NLC, which interlamellar distances increase at higher
temperature. Raman imaging showed that the incorporation of P68 and benzocaine in-between the lipids increased
at higher temperatures, explaining the changes in Q values (SANS). This work shows how different scattering
techniques can provide complementary information and be used together to characterize and understand the
physical, chemical, and structural changes on the organization of pharmaceutical carriers in drug delivery system.
Como referenciar
MITSUTAKE, HERY; SOUZA, ALESSANDRO D. de; YOKAICHIYA, FABIANO; FRANCO, MARGARETH K.K.D.; CLEMENS, DANIEL; KENT, BEN; BREITKREITZ, MARCIA C.; RIBEIRO, LIGIA N. de M.; PAULA, ENEIDA de; BORDALLO, HELOISA N. Evaluation of structural changes of benzocaine-loaded, optimized nanostructured lipid carriers using SANS and Raman imaging approaches. In: CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th, October 4-8, 2021, São Paulo, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq), 2021. p. 183-183. Disponível em: http://repositorio.ipen.br/handle/123456789/32868. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.