The molecular structure of β-alanine is resistant to sterilising doses of gamma radiation
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β-alanine is the rate-limiting point for the endogenous synthesis of carnosine in skeletal
muscle. Carnosine has a wide range of implications for health, normal function and exercise
performance. Whilst the physiological relevance of carnosine to different tissues remains
enigmatic, β-alanine administration is a useful strategy to investigate the physiological roles
of carnosine in humans. Intravenous administration of β-alanine is an interesting approach
to study carnosine metabolism. However, sterilisation is mandatory due to the nature of the
administration route. We evaluated whether sterilising doses of gamma radiation damages
the molecular structure and leads to the loss of functional characteristics of β-alanine. Pure
β-alanine was sterilised by gamma radiation in sealed glass vials using a 60Co multipurpose
irradiator at a dose rate of 8.5 kGy.hour-1 totalising 10, 20, 25 30 and 40 kGy. The molecular
integrity was assessed by X-ray Diffraction and changes in content were determined by
High Performance Liquid Chromatography (UV-HPLC) and Triple Quadrupole Mass Spectrometer
(HPLC/MS-MS). Sterility assurance was evaluated by inoculation assay. To examine
whether functional properties were preserved, β-alanine was infused in one participant,
who rated the level of paraesthesia on the skin using a 0–3 scale. Urinary β-alanine was
quantified before and 24-h following β-alanine infusion using HPLC-ESI+-MS/MS. Irradiation
resulted in no change in the crystal structure of β-alanine, no degradation, and no new
peaks were identified in the dose range assayed. The inoculation assay showed the
absence of viable microorganisms in all β-alanine samples, including those that did not
undergo irradiation. Intravenous infusion of β-alanine resulted in paraesthesia and it
detected in the urine as per normal. We conclude that gamma radiation is a suitable technique
for the sterilisation of β-alanine. It does not lead to degradation, damage to the β-alanine
structure, content or loss of function within the evaluated irradiation conditions.
Como referenciar
GONÇALVES, LIVIA de S.; FRANCHI, MARIANA; MATHOR, MONICA B.; LUGAO, ADEMAR B.; CARVALHO, VICTOR H.; MEDEIROS, MARISA H.G.; ARTIOLI, GUILHERME G.; VARCA, GUSTAVO H.C. The molecular structure of β-alanine is resistant to sterilising doses of gamma radiation. PLoS One, v. 14, n. 1, p. 1-13, 2019. DOI: 10.1371/journal.pone.0210713. Disponível em: http://repositorio.ipen.br/handle/123456789/30069. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.