Development of human fibroblast spheroids with hanging-drop inverted plates

Página do item simplificado
dc.contributor.authorRODRIGUES, ALEX A.
dc.contributor.authorSAMPAIO, MARLOS C.
dc.contributor.authorSANTOS, ESTHER C. dos
dc.contributor.authorPRUDENTE, SULEYNA R.
dc.contributor.authorLIMA, MAYELLE M.P.
dc.contributor.authorSILVA, GIOVANA D. da
dc.contributor.authorMATHOR, MONICA B.
dc.contributor.authorVIEIRA, DANIEL P.
dc.coverageInternacional
dc.creator.eventoLATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th
dc.date.accessioned2024-03-14T19:15:26Z
dc.date.available2024-03-14T19:15:26Z
dc.date.eventoDecember 12-15, 2023
dc.description.abstractIntroduction and objective: 2D cultures have limitations in cell growth. 3D cultures, on the other hand, have become a valuable and powerful tool for biomedical research in recent decades. Due to their resemblance to living systems and cellular interactions, this type of culture can be developed using various methodologies, including nanoparticles, hydrogels, and layers of agarose, among others. Considering the need for testing and validating new molecules and effective therapies for treating various diseases, the objective of this study is to standardize a 3D human fibroblast culture model. Methodology: HF002-J, human fibroblast cells, were cultured at 37ºC in a humid atmosphere containing 5% CO2, maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% antibiotics. When reaching 60- 70% confluence, the cells were detached using a 0.05% trypsin solution. Spheroids were prepared using the hangingdrop technique adapted from [1] 440 µL of medium containing cell variations ranging from 2 × 103 to 6 × 104 cells per well of a 96-well plate were deposited, generating a positive meniscus. The plate was inverted and incubated as described. Results and discussion: The present study aimed to evaluate the development of cellular spheroids after 4 days of culture using different cell preparations. Our results demonstrated that the preparations used produced compact spheroids, characterized by homogeneous sizes in the range of 500 to 1000 μm. When analysing the images obtained by wide-field fluorescence microscopy, we observed that the proportions of unviable cells labelled with fluorophores varied significantly according to the initial number of cells used in the preparations. Notably, increasing the initial number of cells resulted in a proportional increase in the number of non-viable cells present in the formed spheroids. These results suggest that the initial cell density can affect the development and viability of the formed spheroids. It is possible that too high cell density led to greater competition for nutrients and space, resulting in greater cell mortality and less viable spheroids. Conclusions: Based on the results obtained, it was possible to develop an initial prototype of spheroids from human fibroblast cells that can resemble tissues in vivo due to their cellular interactions, thus providing a new tool for the study of drugs and treatments.
dc.event.siglaCOLAOB
dc.format.extent213-213
dc.identifier.citationRODRIGUES, ALEX A.; SAMPAIO, MARLOS C.; SANTOS, ESTHER C. dos; PRUDENTE, SULEYNA R.; LIMA, MAYELLE M.P.; SILVA, GIOVANA D. da; MATHOR, MONICA B.; VIEIRA, DANIEL P. Development of human fibroblast spheroids with hanging-drop inverted plates. In: LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th, December 12-15, 2023, Mar del Plata, Argentina. <b>Abstract...</b> p. 213-213. Disponível em: https://repositorio.ipen.br/handle/123456789/47936.
dc.identifier.orcidhttp://orcid.org/0000-0002-7294-9106
dc.identifier.orcidhttps://orcid.org/0000-0002-0007-534X
dc.identifier.urihttps://repositorio.ipen.br/handle/123456789/47936
dc.local.eventoMar del Plata, Argentina
dc.rightsopenAccess
dc.titleDevelopment of human fibroblast spheroids with hanging-drop inverted plates
dc.typeResumo de eventos científicos
dspace.entity.typePublication
ipen.autorALEX ALVES RODRIGUES
ipen.autorESTHER CAROLINA DOS SANTOS
ipen.autorMAYELLE MARIA PAZ LIMA
ipen.autorGIOVANA DIAS DA SILVA
ipen.autorMONICA BEATRIZ MATHOR
ipen.autorDANIEL PEREZ VIEIRA
ipen.codigoautor15583
ipen.codigoautor15983
ipen.codigoautor14968
ipen.codigoautor15317
ipen.codigoautor209
ipen.codigoautor3158
ipen.contributor.ipenauthorALEX ALVES RODRIGUES
ipen.contributor.ipenauthorESTHER CAROLINA DOS SANTOS
ipen.contributor.ipenauthorMAYELLE MARIA PAZ LIMA
ipen.contributor.ipenauthorGIOVANA DIAS DA SILVA
ipen.contributor.ipenauthorMONICA BEATRIZ MATHOR
ipen.contributor.ipenauthorDANIEL PEREZ VIEIRA
ipen.event.datapadronizada2023
ipen.identifier.ipendoc30279
ipen.notas.internasAbstract
ipen.type.genreResumo
relation.isAuthorOfPublicationb93159e8-8c72-4ac5-bc5a-93911236b814
relation.isAuthorOfPublication9d0ef255-9847-4920-91cc-51c240193f50
relation.isAuthorOfPublication83bccc9e-2167-4216-a6c2-b27567054fdc
relation.isAuthorOfPublication5cd1e045-2814-47b2-b360-caf2443c0464
relation.isAuthorOfPublication742b424f-9dfb-4e4a-993b-000052bb1313
relation.isAuthorOfPublicationc2352608-be9c-4a73-be8c-571f10bb53d2
relation.isAuthorOfPublication.latestForDiscoveryb93159e8-8c72-4ac5-bc5a-93911236b814
sigepi.autor.atividadeRODRIGUES, ALEX A.:15583:810:S
sigepi.autor.atividadeSANTOS, ESTHER C. dos:15983:-1:N
sigepi.autor.atividadeLIMA, MAYELLE M.P.:14968:810:N
sigepi.autor.atividadeSILVA, GIOVANA D. da:15317:810:N
sigepi.autor.atividadeMATHOR, MONICA B.:209:230:N
sigepi.autor.atividadeVIEIRA, DANIEL P.:3158:810:N
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
30279.pdf
Tamanho:
280.99 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar
Coleções
Eventos - Resumos