Iron determination in whole blood samples of dystrophic mice strains using X-ray fluorescence spectrometry
Carregando...
Data
Data de publicação
2017
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
É parte de
RIO SYMPOSIUM ON ATOMIC SPECTROMETRY, 14th
Exportar
Resumo
The use of alternative analytical techniques to investigate specific electrolytes in body fluids has
increased in past years with the goal of adding advantages and simplifications, compared to the
procedures used in conventional clinical practice. Specifically, in this study, a portable X-ray
spectrometer (PXRFS) from Amptek (model X-123 SDD) with Ag X-ray mini-tube has been
employed for the whole blood iron measurements in mice species with Muscular Dystrophy
(Dmdmdx/J, SJL/J and A/J). The term whole blood refers to solid (cells) and liquid (plasma)
components in blood. Usually the conventional procedures for clinical tests are performed in serum
using samples of 0.5 to 1.0 mL. However, when the biological material is scarce (case of small size
animal model, i.e. mice), the possibility to use whole blood became a fascinating alternative for
clinical practice: the Energy Dispersive X-Ray Fluorescence (EDFRX) analyses, using PXRFS,
can be done using 50 μL (the total body blood in mice is around 1.0 mL). In this study, we
investigated whole blood samples of dystrophic mice strains, a genetic disease that cannot be
reversed. In this investigation, twenty samples were investigated. The blood samples were obtained
from Jackson Laboratory (Maine, USA) and further inbred at IPEN. The whole blood was collected
by the retro–orbital venous plexus and immediately transferred to filter paper (Whatman n41),
prior to its coagulation. No anticoagulants or reagents have been used to prevent interference on
the results. This procedure simplify the preparation and conservation of biological samples: it is
not a destructive procedure and the sample does not need refrigeration. The collection was
performing according to the rules approved by Animal Research Ethics Committee (087/99). A
comparative study between EDXFX and INAA (Instrumental Neutron Activation Analysis) data
was also carried out and the results are in good agreement. This alternative procedure was capable,
in a few minutes, to determine whole blood Fe concentrations higher than 6.5 mgL-1, without
chemical digestion using direct and non-destructive analysis. The study of Fe behavior in whole
blood samples of mice with muscular dystrophy contributes to its diagnosis.
Como referenciar
SILVA, DALTON G.N. da; METAIRON, SABRINA; ZAMBONI, CIBELE B.; SUZUKI, MIRIAM F.; BUENO JUNIOR, CARLOS R.; RIZZUTTO, MARCIA de A. Iron determination in whole blood samples of dystrophic mice strains using X-ray fluorescence spectrometry. In: RIO SYMPOSIUM ON ATOMIC SPECTROMETRY, 14th, 02-07 de abril, 2017, Vitória, ES. Abstract... Disponível em: http://repositorio.ipen.br/handle/123456789/27660. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.