Radiation effects on Toxoplasma antigens

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dc.contributor.authorCOSTA, ANDREA dapt_BR
dc.contributor.authorNASCIMENTO, NANCI dopt_BR
dc.contributor.authorGALISTEO JUNIOR, ANDRES J.pt_BR
dc.contributor.authorPASSOS, ALINE B.D. dospt_BR
dc.contributor.authorANDRADE JUNIOR, HEITOR F. dept_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2020-12-21T19:22:07Z
dc.date.available2020-12-21T19:22:07Z
dc.date.issued2020pt_BR
dc.description.abstractPurpose: Purpose: Protein irradiation causes aggregation, chain breakage, and oxidation, enhancing its uptake by antigen-presenting cells. To evaluate if irradiated proteins participate on the protection, we studied the immune response induced in mice immunized with irradiated soluble extracts of T. gondii tachyzoites (STag) or irradiated intact T. gondii RH tachyzoites (RH0.25 kGy). Material and Methods: Soluble extracts of Toxoplasma gondii tachyzoites (STag) were irradiated at different dose by Cobalt-60 source. By polyacrylamide gel electrophoresis (SDS-Page) we evaluated the effects on primary structures of protein STags induced by irradiation. By Enzyme-linked Immunosorbent Assay (ELISA) we evaluated the difference between humoral immune response induced by irradiated STag or RH tachyzoites in immunized mice from the detection of specific immunoglobulin G (IgG) antibodies in the serum of immunized mice. From challenge with viable RH strain of T. gondii we evaluated the protection induced in the immunized animals. By cytometry we performed the phenotyping of T and B lymphocytes in the peripheral blood of the immunized animals. Results: Irradiation dose of 1.5 kGy induced minimal changes in most proteins, without affecting their antigenicity or immunogenicity. Immunization showed saturation at the dose of 10 µg/mice, with worst response at higher doses. STag irradiated at 1.5  kGy (STag1.5 kGy) induced higher survival and protection similar to T. gondii RH strain irradiated at 0.25 kGy (RH0.25 kGy), with higher serum levels of high affinity IgG compared to STag native. Blood immune memory cells of mice immunized with STag1.5 kGy had higher proportions of CD19+ (cluster of differentiation 19) and CD4+ (cluster of differentiation 14) cells, whereas mice RH0.25 kGy had high proportion of memory CD8+ (cluster of differentiation 8) cells. Conclusions: Our data suggest that major histocompatibility complex type I (MHCI) pathway, appears seem to be used by RH0.25 kGy to generate cytotoxic cells while STag1.5 kGy uses a major histocompatibility complex type II (MHCII) pathway for B-cell memory, but both induce sufficient immune response for protection in mice without any adjuvant. Irradiation of soluble protein extracts enhances their immune response, allowing similar protection against T. gondii in mice as compared to irradiated intact parasites.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipIDFAPESP: 14/17029-4pt_BR
dc.description.sponsorshipIDCAPES: 001pt_BR
dc.format.extent697-706pt_BR
dc.identifier.citationCOSTA, ANDREA da; NASCIMENTO, NANCI do; GALISTEO JUNIOR, ANDRES J.; PASSOS, ALINE B.D. dos; ANDRADE JUNIOR, HEITOR F. de. Radiation effects on Toxoplasma antigens: different immune responses of irradiated intact tachyzoites or soluble antigens in experimental mice models. <b>International Journal of Radiation Biology</b>, v. 96, n. 5, p. 697-706, 2020. DOI: <a href="https://dx.doi.org/10.1080/09553002.2020.1704298">10.1080/09553002.2020.1704298</a>. Disponível em: http://200.136.52.105/handle/123456789/31699.
dc.identifier.doi10.1080/09553002.2020.1704298pt_BR
dc.identifier.fasciculo5pt_BR
dc.identifier.issn0955-3002pt_BR
dc.identifier.percentilfi64.74pt_BR
dc.identifier.percentilfiCiteScore71.00
dc.identifier.urihttp://200.136.52.105/handle/123456789/31699
dc.identifier.vol96pt_BR
dc.relation.ispartofInternational Journal of Radiation Biologypt_BR
dc.rightsopenAccesspt_BR
dc.subjectprotozoa
dc.subjectparasites
dc.subjectparasitic diseases
dc.subjectgamma radiation
dc.subjectradiation effects
dc.subjectvaccines
dc.subjectimmunity
dc.subjectionizing radiations
dc.subjectcobalt 60
dc.subjectantigens
dc.subjectmice
dc.titleRadiation effects on Toxoplasma antigenspt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorNANCI DO NASCIMENTO
ipen.codigoautor411
ipen.contributor.ipenauthorNANCI DO NASCIMENTO
ipen.date.recebimento20-12
ipen.identifier.fi2.694pt_BR
ipen.identifier.fiCiteScore4.2
ipen.identifier.ipendoc27471pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi1.500 - 2.999
ipen.range.percentilfi50.00 - 74.99
ipen.subtitulodifferent immune responses of irradiated intact tachyzoites or soluble antigens in experimental mice modelspt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublication53eed426-0bd7-4230-8b8c-f48795fad345
relation.isAuthorOfPublication.latestForDiscovery53eed426-0bd7-4230-8b8c-f48795fad345
sigepi.autor.atividadeNASCIMENTO, NANCI do:411:-1:Npt_BR
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