Disposable electrochemical microfluidic device for ultrasensitive detection of egg allergen in wine samples

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dc.contributor.authorBALDO, THAISA A.pt_BR
dc.contributor.authorPROENCA, CAMILA dos A.pt_BR
dc.contributor.authorFELIX, FABIANA da S.pt_BR
dc.contributor.authorFREITAS, TAYANE A.pt_BR
dc.contributor.authorSAKATA, SOLANGE K.pt_BR
dc.contributor.authorANGNES, LUCIOpt_BR
dc.contributor.authorFARIA, RONALDO C.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2021-12-13T13:36:36Z
dc.date.available2021-12-13T13:36:36Z
dc.date.issued2021pt_BR
dc.description.abstractFood allergies have been increasing all over the world. Egg is an important component in the food industries and the second most common cause of food allergy, shortly after milk. In the wine industry, egg white is applied as a fining agent for tannin removal. In this study, a sandwich-based immunoassay for ultrasensitive detection of ovalbumin (OVA) in wine samples was developed. The assay involves the use of magnetic beads (MBs) decorated with a polyclonal anti-OVA antibody (Ab2) and horseradish peroxidase (HRP), used as label for the quantification in a disposable electrochemical microfluidic device (DEμD) here developed. The Ab2-MB-HRP prepared was applied to capture, separate, and pre-concentrate OVA from wine samples. In the DEμD, OVA was immune-magnetically captured (OVA-Ab2-MB-HRP), producing a sandwich structure (GO-Ab1-OVA-Ab2-MB-HRP) on the electrode's surface. This arrangement results in an ultrasensitive device, achieving the ultralow limit of detection of 0.2 fg mL−1 OVA. Five samples of wines were analyzed by using the immuno-magneto-assay which presents excellent accuracy compared with enzyme-linked immunosorbent assay (ELISA).pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDCAPES: 001pt_BR
dc.description.sponsorshipIDCNPq: 308570/2018-9; 311847/2018-8pt_BR
dc.description.sponsorshipIDFAPESP: 13/14262-7; 16/00991-5; 17/24053-7; 17/13137-5; 14/50867-3pt_BR
dc.format.extent1-9pt_BR
dc.identifier.citationBALDO, THAISA A.; PROENCA, CAMILA dos A.; FELIX, FABIANA da S.; FREITAS, TAYANE A.; SAKATA, SOLANGE K.; ANGNES, LUCIO; FARIA, RONALDO C. Disposable electrochemical microfluidic device for ultrasensitive detection of egg allergen in wine samples. <b>Talanta</b>, v. 232, p. 1-9, 2021. DOI: <a href="https://dx.doi.org/10.1016/j.talanta.2021.122447">10.1016/j.talanta.2021.122447</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/32396.
dc.identifier.doi10.1016/j.talanta.2021.122447pt_BR
dc.identifier.issn0039-9140pt_BR
dc.identifier.orcid0000-0001-6072-5853pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-6072-5853
dc.identifier.percentilfi87.93pt_BR
dc.identifier.percentilfiCiteScore89.00pt_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/32396
dc.identifier.vol232pt_BR
dc.relation.ispartofTalantapt_BR
dc.rightsopenAccesspt_BR
dc.subjecteggs
dc.subjectallergy
dc.subjectovalbumin
dc.subjectenzyme immunoassay
dc.subjectbeverages
dc.subjectantigens
dc.titleDisposable electrochemical microfluidic device for ultrasensitive detection of egg allergen in wine samplespt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorSOLANGE KAZUMI SAKATA
ipen.codigoautor7038
ipen.contributor.ipenauthorSOLANGE KAZUMI SAKATA
ipen.date.recebimento21-12
ipen.identifier.fi6.556pt_BR
ipen.identifier.fiCiteScore10.6pt_BR
ipen.identifier.ipendoc28164pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi6.000 ou mais
ipen.range.percentilfi75.00 - 100.00
ipen.type.genreArtigo
relation.isAuthorOfPublicationfb62f288-104d-4352-aed0-a18e0da21a1b
relation.isAuthorOfPublication.latestForDiscoveryfb62f288-104d-4352-aed0-a18e0da21a1b
sigepi.autor.atividadeSAKATA, SOLANGE K.:7038:220:Npt_BR
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