Rational selection of hidden epitopes for a molecularly imprinted electrochemical sensor in the recognition of heat-denatured dengue NS1 protein

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dc.contributor.authorSILVA, MATHEUS S.pt_BR
dc.contributor.authorTAVARES, ANA P.M.pt_BR
dc.contributor.authorCOELHO, LUIZ F.L.pt_BR
dc.contributor.authorDIAS, LIGIA E.M.F.pt_BR
dc.contributor.authorCHURA-CHAMBI, ROSA M.pt_BR
dc.contributor.authorFONSECA, FLAVIO G. dapt_BR
dc.contributor.authorSALES, MARIA G.F.pt_BR
dc.contributor.authorFIGUEIREDO, EDUARDO C.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2022-01-25T12:02:52Z
dc.date.available2022-01-25T12:02:52Z
dc.date.issued2021pt_BR
dc.description.abstractRational selection of predicted peptides to be employed as templates in molecular imprinting was carried out for the heat-denatured non-structural protein 1 (NS1) of dengue virus (DENV). Conservation analysis among 301 sequences of Brazilian isolates of DENV and zika virus (ZIKV) NS1 was carried out by UniProtKB, and peptide selection was based on in silico data of the conservational, structural and immunogenic properties of the sequences. The selected peptide (from dengue 1 NS1) was synthesized and employed as a template in the electropolymerization of polyaminophenol-imprinted films on the surface of carbon screen-printed electrodes. Heat denaturation of the protein was carried out prior to analysis, in order to expose its internal hidden epitopes. After removal of the template, the molecularly imprinted cavities were able to rebind to the whole denatured protein as determined by electrochemical impedance spectroscopy. This label-free sensor was efficient to distinguish the NS1 of DENV from the NS1 of ZIKV. Additionally, the sensor was also selective for dengue NS1, in comparison with human serum immunoglobulin G and human serum albumin. Additionally, the device was able to detect the DENV NS1 at concentrations from 50 to 200 μg L−1 (RSD below 5.04%, r = 0.9678) in diluted human serum samples. The calculated LOD and LOQ were, respectively, 29.3 and 88.7 μg L−1 and each sensor could be used for six sequential cycles with the same performance.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipIDFAPEMIG: CDS-APQ-00638-17pt_BR
dc.description.sponsorshipIDCNPq: 301564/2019-1; 427365/2018-0pt_BR
dc.description.sponsorshipIDCAPES: 88881.133746/2016-01pt_BR
dc.format.extent1-8pt_BR
dc.identifier.citationSILVA, MATHEUS S.; TAVARES, ANA P.M.; COELHO, LUIZ F.L.; DIAS, LIGIA E.M.F.; CHURA-CHAMBI, ROSA M.; FONSECA, FLAVIO G. da; SALES, MARIA G.F.; FIGUEIREDO, EDUARDO C. Rational selection of hidden epitopes for a molecularly imprinted electrochemical sensor in the recognition of heat-denatured dengue NS1 protein. <b>Biosensors and Bioelectronics</b>, v. 191, p. 1-8, 2021. DOI: <a href="https://dx.doi.org/10.1016/j.bios.2021.113419">10.1016/j.bios.2021.113419</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/32642.
dc.identifier.doi10.1016/j.bios.2021.113419pt_BR
dc.identifier.issn0956-5663pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7870-1793
dc.identifier.percentilfi93.02pt_BR
dc.identifier.percentilfiCiteScore97.00pt_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/32642
dc.identifier.vol191pt_BR
dc.relation.ispartofBiosensors and Bioelectronicspt_BR
dc.rightsopenAccesspt_BR
dc.subjectviral diseases
dc.subjectviral diseases
dc.subjectzika virus
dc.subjectprotein denaturation
dc.subjectpolymers
dc.subjectsensors
dc.titleRational selection of hidden epitopes for a molecularly imprinted electrochemical sensor in the recognition of heat-denatured dengue NS1 proteinpt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorROSA MARIA CHURA CHAMBI
ipen.autorLIGIA ELY MORGANTI FERREIRA DIAS
ipen.codigoautor3338
ipen.codigoautor296
ipen.contributor.ipenauthorROSA MARIA CHURA CHAMBI
ipen.contributor.ipenauthorLIGIA ELY MORGANTI FERREIRA DIAS
ipen.date.recebimento22-01
ipen.identifier.fi12.545pt_BR
ipen.identifier.fiCiteScore20.2pt_BR
ipen.identifier.ipendoc28410pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.identifier.ods3
ipen.range.fi6.000 ou mais
ipen.range.percentilfi75.00 - 100.00
ipen.type.genreArtigo
relation.isAuthorOfPublicationd2b97487-e004-4056-8df7-0ab5c8d5cacc
relation.isAuthorOfPublicationc26a78ae-be8e-4a8d-b22c-0a62eb422f8b
relation.isAuthorOfPublication.latestForDiscoveryc26a78ae-be8e-4a8d-b22c-0a62eb422f8b
sigepi.autor.atividadeCHURA-CHAMBI, ROSA M.:3338:810:Npt_BR
sigepi.autor.atividadeDIAS, LIGIA E.M.F.:296:810:Npt_BR
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