Development of spheroid in co-culture of prostate tumor with human fibroblast, using the hanging drop technique with plate inversion for analysis of GammaCell220 irradiation in Co-60

Abstract

The development of tumor spheroids in co-culture with human fibroblasts represents an innovative approach to modeling the prostate cancer microenvironment and assessing radiation effects. In this study, we employed the "hanging drop" technique in conjunction with plate inversion to generate three-dimensional spheroids composed of prostate tumor cells (LNCAP cell line) co-cultivated with human fibroblasts. This approach aims to create a more representative in vivo-like tumor environment, facilitating a detailed analysis of the interactions between tumor cells and fibroblasts in 3D conditions. The formed spheroids were exposed to gamma radiation using a GammaCell 220 irradiator with Co-60, with the goal of investigating the effects of radiation on both fibroblastic and tumor cells. Gamma radiation is known to induce cell death, and this study examines how such damage impacts cellular morphology within the spheroid microenvironment.