Sterilization of chitosan nanogels
Carregando...
Data
Data de publicação
2017
Autores IPEN
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
É parte de
BRAZIL MRS MEETING, 16th
Exportar
Resumo
A nanosystem designed for biomedical applications (e.g. injectables, ophthalmic
solutions) cannot be toxic or irritating and must be sterile [1]. Therefore,
sterilization is a critical but needed step, during which adverse effects can occur.
Literature is very scarce with respect to sterilization effects on nanostructured
systems, and even more in what concerns nanogels [1]. This work aims to
evaluate the effect and effectiveness of conventional terminal sterilization
methods (steam heat and gamma irradiation) on chitosan hydrogel nanoparticles.
Chitosan-tripolyphosphate based hydrogel nanoparticles (CS/TPP), with broad
spectrum of possible applications were produced and sterilized in the absence
and in the presence of protective sugars (glucose and mannitol). Properties like
size, zeta potential, absorbance, morphology, chemical structure and cytotoxicity
were evaluated. The obtained results allowed concluding that steam heat is not a
suitable method for sterilizing CS/TPP nanogel as it leads to severe degradation of
the samples, with the appearance of multiple particle populations of different
sizes, and large aggregates and sediments. Gamma rays exposure gave rise to
immediate formation of visible sediments. However, upon the addition of
protective sugars (glucose and mannitol 5%) a significant increase of the nanogel
resistance to radiation was observed. This protective effect could be related with
the formation of an antioxidant complex.
Como referenciar
GALANTE, RAQUEL; REDIGUIERI, CAROLINA F.; KIKUCHI, IRENE S.; VASQUEZ, PABLO; COLAÇO, ROGERIO; SERRO, ANA P.; PINTO, TEREZINHA de J.A. Sterilization of chitosan nanogels: the protective role of sugars. In: BRAZIL MRS MEETING, 16th, September 10-14, 2017, Gramado, RS. Abstract... São Carlos, SP: Aptor Software, 2017. p. 1720-1720. Disponível em: http://repositorio.ipen.br/handle/123456789/31201. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.