Immobilized kidney 28 KDa endostatin-related (KES28KDa) fragment promotes endothelial cell survival

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dc.contributor.authorBELLINI, MARIA H.pt_BR
dc.contributor.authorMALPIGHI, THIAGO F.pt_BR
dc.contributor.authorCALVO, FERNANDA B.pt_BR
dc.contributor.authorMIRANDA, ADRIANA R.pt_BR
dc.contributor.authorSPENCER, PATRICK J.pt_BR
dc.contributor.authorCICHY, MILENA C.pt_BR
dc.contributor.authorSIMONS, SIMONE M.pt_BR
dc.contributor.authorTAVASSI, ANA M.C.pt_BR
dc.contributor.authorSANTOS, MARINILCE F. dospt_BR
dc.contributor.authorRODRIGUES, CONSUELO J.pt_BR
dc.contributor.authorSCHOR, NESTORpt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2014-07-31T11:35:29Zpt_BR
dc.date.accessioned2014-07-31T11:50:54Z
dc.date.available2014-07-31T11:35:29Zpt_BR
dc.date.available2014-07-31T11:50:54Z
dc.date.issued2010pt_BR
dc.description.abstractBackground/Objective: Renal ischemia-hypoxia is a leading cause of acute kidney injury (AKI). Ischemia causes extracellular matrix breakdown of the tubular basement membrane. Endostatin (ES) is the C-terminal fragment of collagen XVIII generated by proteolytic cleavage. Recent studies have demonstrated that ES expression is upregulated in ischemic kidneys. The present study aimed to characterize ES from ischemic kidneys. Methods: Ischemic renal failure was induced via 45 min of occlusion of the left renal artery and vein. After the ischemic period, blood was collected. Kidneys were harvested and used for immunohistochemical testing and protein extraction. Three-step purification was used. Soluble and immobilized purified ES were tested in cell viability and adhesion assays. Results: The soluble KES28kDa inhibited endothelial cell proliferation: 25 versus 12.5 g (p ! 0.05); 12.5 versus 3.15 g (p ! 0.05). Immobilization of KES28kDa supports endothelial cell survival over the control (p = 0.021). Human umbilical vein endothelial cells plated on immobilized KES28kDa showed an increase in membrane ruffles and stress fibers. Conclusion: These data demonstrate the local synthesis of a 28-kDa ES-related fragment following AKI and suggest its role in endothelium survival.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDFAPESP:07/51204-4pt_BR
dc.format.extent255-261pt_BR
dc.identifier.citationBELLINI, MARIA H.; MALPIGHI, THIAGO F.; CALVO, FERNANDA B.; MIRANDA, ADRIANA R.; SPENCER, PATRICK J.; CICHY, MILENA C.; SIMONS, SIMONE M.; TAVASSI, ANA M.C.; SANTOS, MARINILCE F. dos; RODRIGUES, CONSUELO J.; SCHOR, NESTOR. Immobilized kidney 28 KDa endostatin-related (KES28KDa) fragment promotes endothelial cell survival. <b>American Journal of Nephrology</b>, v. 31, n. 3, p. 255-261, 2010. DOI: <a href="https://dx.doi.org/10.1159/000278756">10.1159/000278756</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/8153.
dc.identifier.doi10.1159/000278756
dc.identifier.fasciculo3pt_BR
dc.identifier.issn0250-8095pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8949-7735
dc.identifier.orcidhttps://orcid.org/0000-0003-2852-6189
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/8153pt_BR
dc.identifier.vol31pt_BR
dc.relation.ispartofAmerican Journal of Nephrologypt_BR
dc.rightsopenAccessen
dc.subjectkidneyspt_BR
dc.subjectinjuriespt_BR
dc.subjectischemiapt_BR
dc.subjectanimal tissuespt_BR
dc.subjectendotheliumpt_BR
dc.subjectpromoterspt_BR
dc.subjectimmune system diseasespt_BR
dc.subjecttestingpt_BR
dc.titleImmobilized kidney 28 KDa endostatin-related (KES28KDa) fragment promotes endothelial cell survivalpt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorTHIAGO FRANCA MALPIGHI SANTOS
ipen.autorADRIANA REGINA MIRANDA
ipen.autorPATRICK JACK SPENCER
ipen.autorFERNANDA BERNARDES CALVO
ipen.autorMARIA HELENA BELLINI MARUMO
ipen.codigoautor8241
ipen.codigoautor8695
ipen.codigoautor910
ipen.codigoautor3896
ipen.codigoautor1242
ipen.contributor.ipenauthorTHIAGO FRANCA MALPIGHI SANTOS
ipen.contributor.ipenauthorADRIANA REGINA MIRANDA
ipen.contributor.ipenauthorPATRICK JACK SPENCER
ipen.contributor.ipenauthorFERNANDA BERNARDES CALVO
ipen.contributor.ipenauthorMARIA HELENA BELLINI MARUMO
ipen.date.recebimento10-12pt_BR
ipen.identifier.fi2.658pt_BR
ipen.identifier.ipendoc16101pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi1.500 - 2.999
ipen.type.genreArtigo
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relation.isAuthorOfPublication.latestForDiscovery6a450cbf-91b1-4386-b4a8-7304afac99cc
sigepi.autor.atividadeBELLINI, MARIA H.:1242:820:Spt_BR
sigepi.autor.atividadeMALPIGHI, THIAGO F.:8241:820:Npt_BR
sigepi.autor.atividadeCALVO, FERNANDA B.:3896:-1:Npt_BR
sigepi.autor.atividadeMIRANDA, ADRIANA R.:8695:820:Npt_BR
sigepi.autor.atividadeSPENCER, PATRICK J.:910:820:Npt_BR
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