Nanoencapsulation of anthocyanins based on pectin and lysozyme

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2024
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INTERNATIONAL CONFERENCE ON FOOD DIGESTION, 8th
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Anthocyanins are bioactive compounds with essential properties, such as antioxidant, anti-inflammatory, and anticancer. However, they have high molecular instability, limited bioaccessibility, and bioavailability. The study aimed to develop a new methodology to nano-encapsulate anthocyanins extracted from blackberries (Rubus spp.) using nanogels. They were based on citrus pectin associated with lysozyme through molecular self-assembling. Nanoparticles were subjected to evaluation of colloidal stability and behavior on in vitro and in vivo models. Cyanidin-3-O-glycoside was primarily identified as the main anthocyanin (95%), and the nanoencapsulation proportions of 1:2:0.4 (m:m:m) of pectin, lysozyme, and anthocyanin, respectively, were statistically established. The nanoparticles showed a size of 190 nm, Zeta Potential -30 mV, and invariably spherical and homogeneous morphology (polydispersity index of 0.1). Nano-encapsulated anthocyanins were stable at different pH values (2 to 12) and temperatures (4, 25 and 40°C). The INFOGEST 2.0 digestion indicated preserving the anthocyanins' integrity and gradual release. A significant content remained intact in the nanostructure at the end of the intestinal phase. Cytotoxicity was not observed in 2D and 3D models, and nanoparticles were absorbed in both cell systems in a time-dependent way. A new methodology was developed to radiolabel anthocyanins directly with Technetium (99mTc) and nano-encapsulate them. An in silico model was developed to indicate the molecular interaction between the anthocyanin and the radioisotope. Biodistribution in different tissues, Kinect of absorption, and molecular visualization by μSPECT/CT showed that nano-encapsulated 99mTc-anthocyanins are absorbed differently than free molecules in mice. The molecule in blood, bone, bladder, lung, brain, heart, pancreas, liver, and spleen was significantly higher for nano-encapsulated after 24 hours of oral administration. The in vitro and in vivo methodologies demonstrated that nano-encapsulation can protect anthocyanins against digestion factors, with a controlled release in the intestine, improving absorption and targeting different organs. These results contribute to knowledge about the bioavailability of nano-encapsulated bioactive compounds and their potential clinical applications for the food and pharmaceutical industries.

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OSVALDT ROSALES, THIECLA K.; ALVES DA SILVA, FABIO F.; GONZALEZ RIVERA, ANDY; SOARES BERNARDES, EMERSON; FABI, JOAO P. Nanoencapsulation of anthocyanins based on pectin and lysozyme: a new technological approach to increase the physicochemical stability, bioaccessibility and absorption. In: INTERNATIONAL CONFERENCE ON FOOD DIGESTION, 8th, April 9-11, 2024, Porto, Portugal. Abstract... Disponível em: https://repositorio.ipen.br/handle/123456789/48142. Acesso em: 22 Jul 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.

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