The amphibian diacylglycerol O-acyltransferase 2 (DGAT2)
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2019
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Protein Journal
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Amphibians are, currently, considered the first vertebrates that had performed the aquatic to terrestrial transition during evolution;
therefore, water balance and dehydration control were prerequisites for such environment conquering. Among anurans,
Phyllomedusa is a well-studied genus, due to its peptide-rich skin secretion. Here, we have analyzed the skin secretion of
Phyllomedusa distincta targeting the proteins present in the skin secretion. The major soluble protein was chromatographically
isolated and utilized to immunize rabbits. Through proteomics approaches, we were able to identify such protein as
being the diacylglycerol O-acyltransferase 2 (DGAT2), a crucial enzyme involved in lipid synthesis and in the skin water
balance. Immunohistochemistry assays revealed the protein tissular distribution for different animal species, belonging to
different branches of the phylogenetic tree. Specifically, there was positivity to the anti-DGAT2 on Amphibians’ skin, and
no antibody recognition on fish and mammals’ skins. The DGAT2 multiple sequence alignment reveals some degree of
conservation throughout the genera; however, there is a different cysteine pattern among them. Molecular modeling analyses
corroborate that the different cysteine pattern leads to distinct 3D structures, explaining the different antibody recognition.
Moreover, the protein phylogenetic analyses place the Xenopus DGAT2 (the available amphibian representative) next to the
Coelacanthus enzyme, which have led the authors to term this a ‘paleo-protein’. DGAT2 would be, therefore, an ancient
protein, crucial to the terrestrial environment conquest, with a unique folding—as indicated by the molecular models and
immunohistochemistry analyses—a consequence of the different cysteine pattern but with conserved biological function.
Como referenciar
SCIANI, JULIANA M.; NEVES, ADRIANA; VASSÃO, RUTH C.; SPENCER, PATRICK; ANTONIAZZI, MARTA M.; JARED, CARLOS; PIMENTA, DANIEL C. The amphibian diacylglycerol O-acyltransferase 2 (DGAT2): a ‘paleoprotein’ with conserved function but unique folding. Protein Journal, v. 38, n. 1, p. 83-94, 2019. DOI: 10.1007/s10930-019-09814-x. Disponível em: http://repositorio.ipen.br/handle/123456789/30037. Acesso em: 06 Oct 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.