Evaluation of radioinduced damage and repair capacity in blood lymphocytes of breast cancer patients

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dc.contributor.authorNASCIMENTO, P.A.pt_BR
dc.contributor.authorSILVA, M.A.pt_BR
dc.contributor.authorOLIVEIRA, E.M.pt_BR
dc.contributor.authorSUZUKI, M.F.pt_BR
dc.contributor.authorOKAZAKI, K.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2014-07-30T17:22:38Zpt_BR
dc.date.accessioned2014-07-30T19:00:10Z
dc.date.available2014-07-30T17:22:38Zpt_BR
dc.date.available2014-07-30T19:00:10Z
dc.date.issued2001pt_BR
dc.description.abstractGenetic damage caused by ionizing radiation and repair capacity of blood lymphocytes from 3 breast cancer patients and 3 healthy donors were investigated using the comet assay. The comets were analyzed by two parameters: comet tail length and visual classification. Blood samples from the donors were irradiated in vitro with a 60Co source at a dose rate of 0.722 Gy/min, with a dose range of 0.2 to 4.0 Gy and analyzed immediately after the procedure and 3 and 24 h later. The basal level of damage and the radioinduced damage were higher in lymphocytes from breast cancer patients than in lymphocytes from healthy donors. The radioinduced damage showed that the two groups had a similar response when analyzed immediately after the irradiations. Therefore, while the healthy donors presented a considerable reduction of damage after 3 h, the patients had a higher residual damage even 24 h after exposure. The repair capacity of blood lymphocytes from the patients was slower than that of lymphocytes from healthy donors. The possible influence of age, disease stage and mutations in the BRCA1 and BRCA2 genes are discussed. Both parameters adopted proved to be sensitive and reproducible: the dose-response curves for DNA migration can be used not only for the analysis of cellular response but also for monitoring therapeutic interventions. Lymphocytes from the breast cancer patients presented an initial radiosensitivity similar to that of healthy subjects but a deficient repair mechanism made them more vulnerable to the genotoxic action of ionizing radiation. However, since lymphocytes from only 3 patients and 3 normal subjects were analyzed in the present paper, additional donors will be necessary for a more accurate evaluation.
dc.format.extent165-176pt_BR
dc.identifier.citationNASCIMENTO, P.A.; SILVA, M.A.; OLIVEIRA, E.M.; SUZUKI, M.F.; OKAZAKI, K. Evaluation of radioinduced damage and repair capacity in blood lymphocytes of breast cancer patients. <b>Brazilian Journal of Medical and Biological Research</b>, v. 34, n. 2, p. 165-176, 2001. DOI: <a href="https://dx.doi.org/10.1590/S0100-879X2001000200003">10.1590/S0100-879X2001000200003</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/6207.
dc.identifier.doi10.1590/S0100-879X2001000200003
dc.identifier.fasciculo2pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/6207pt_BR
dc.identifier.vol34pt_BR
dc.relation.ispartofBrazilian Journal of Medical and Biological Researchpt_BR
dc.rightsopenAccessen
dc.subjectbiological radiation effectspt_BR
dc.subjectlymphocytespt_BR
dc.subjectmammary glandspt_BR
dc.subjectneoplasmspt_BR
dc.subjectdna repairpt_BR
dc.subjectstrand breakspt_BR
dc.subjectradiosensitivitypt_BR
dc.subjectelectrophoresispt_BR
dc.subjectgamma radiationpt_BR
dc.subjectcobalt 60pt_BR
dc.titleEvaluation of radioinduced damage and repair capacity in blood lymphocytes of breast cancer patientspt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorKAYO OKAZAKI
ipen.autorMIRIAM FUSSAE SUZUKI
ipen.autorELAINE MENDES DE OLIVEIRA
ipen.autorPATRICIA ALVES DO NASCIMENTO
ipen.autorMARCIA AUGUSTA DA SILVA
ipen.codigoautor1220
ipen.codigoautor557
ipen.codigoautor2407
ipen.codigoautor2057
ipen.codigoautor2589
ipen.contributor.ipenauthorKAYO OKAZAKI
ipen.contributor.ipenauthorMIRIAM FUSSAE SUZUKI
ipen.contributor.ipenauthorELAINE MENDES DE OLIVEIRA
ipen.contributor.ipenauthorPATRICIA ALVES DO NASCIMENTO
ipen.contributor.ipenauthorMARCIA AUGUSTA DA SILVA
ipen.date.recebimento00-12pt_BR
ipen.identifier.fiSem F.I.pt_BR
ipen.identifier.ipendoc06655pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.type.genreArtigo
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sigepi.autor.atividadeNASCIMENTO, P.A.:2057:-1:Spt_BR
sigepi.autor.atividadeSILVA, M.A.:2589:-1:Npt_BR
sigepi.autor.atividadeOLIVEIRA, E.M.:2407:-1:Npt_BR
sigepi.autor.atividadeSUZUKI, M.F.:557:28:Npt_BR
sigepi.autor.atividadeOKAZAKI, K.:1220:28:Npt_BR
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