MicroRNA expression profile in head and neck cancer
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Abstract
Background: Current evidence implicates aberrant microRNA expression patterns in human malignancies;
measurement of microRNA expression may have diagnostic and prognostic applications. Roles for microRNAs in
head and neck squamous cell carcinomas (HNSCC) are largely unknown. HNSCC, a smoking-related cancer, is one
of the most common malignancies worldwide but reliable diagnostic and prognostic markers have not been discovered so far. Some studies have evaluated the potential use of microRNA as biomarkers with clinical application
in HNSCC.
Methods: MicroRNA expression profile of oral squamous cell carcinoma samples was determined by means of DNA
microarrays. We also performed gain-of-function assays for two differentially expressed microRNA using two
squamous cell carcinoma cell lines and normal oral keratinocytes. The effect of the over-expression of these
molecules was evaluated by means of global gene expression profiling and cell proliferation assessment.
Results: Altered microRNA expression was detected for a total of 72 microRNAs. Among these we found well
studied molecules, such as the miR-17-92 cluster, comprising potent oncogenic microRNA, and miR-34, recently
found to interact with p53. HOX-cluster embedded miR-196a/b and miR-10b were up- and down-regulated,
respectively, in tumor samples. Since validated HOX gene targets for these microRNAs are not consistently
deregulated in HNSCC, we performed gain-of-function experiments, in an attempt to outline their possible role. Our
results suggest that both molecules interfere in cell proliferation through distinct processes, possibly targeting a small
set of genes involved in cell cycle progression.
Conclusions: Functional data on miRNAs in HNSCC is still scarce. Our data corroborate current literature and
brings new insights into the role of microRNAs in HNSCC. We also show that miR-196a and miR-10b, not previously
associated with HNSCC, may play an oncogenic role in this disease through the deregulation of cell proliferation. The
study of microRNA alterations in HNSCC is an essential step to the mechanistic understanding of tumor formation and
could lead to the discovery of clinically relevant biomarkers.
Como referenciar
SEVERINO, PATRICIA; BRUGGEMANN, HOLGER; ANDREGHETTO, FLAVIA M.; CAMPS, CARME; KLINGBEIL, MARIA de F.G.; PEREIRA, WELBERT O. de; SOARES, RENATA M.; MOYSES, RAQUEL; WUNSCH FILHO, VICTOR; MATHOR, MONICA B.; NUNES, FABIO D.; RAGOUSSIS, JIANNIS; TAJARA, ELOIZA H. MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a and microRNA-10b dysregulation implicated in cell proliferation. BMC Cancer, v. artigo 533, 2013. DOI: 10.1186/1471-2407-13-533. Disponível em: http://repositorio.ipen.br/handle/123456789/8925. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.