Evaluation of the antiangiogenic activity of zinc in renal adenocarcinoma in a hypoxic microenvironment
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2024
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ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 53rd
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Renal cancer is a common urologic malignancy, and therapeutic options for metastatic disease are limited. Most clear
cell renal cell carcinomas (ccRCC) are associated with loss of Von Hippel-Lindau tumor suppressor (VHL) function and
deregulation of hypoxia pathways. This deregulation induces angiogenesis, promoting tumor growth and metastasis.
Vascular endothelial growth factor (VEGF), a pro-angiogenic factor, is overexpressed in ccRCC, contributing to its
aggressive nature. Zinc (Zn), an essential micronutrient, has garnered attention for its potential in cancer therapy. While
Zn deficiency increases cancer risk, Zn supplementation exhibits anticancer effects, inhibiting proliferation and
migration of some types of cancer. However, conflicting evidence regarding Zn's association with RCC necessitates
further research to elucidate its role in renal carcinogenesis. This study aimed to evaluate the effect of Zinc Chloride
on angiogenesis regulator genes in ccRCC under normoxic and hypoxic conditions. The MTS (3-(4,5-dimethylthiazol-
2-yl)-2,5-diphenyltetrazolium-bromide) assay was used to define cell viability of different Zn concentrations on ccRCC
cells. The expression of HIF-1a and VEGF-a genes from the 786-0, 786-0 + Zn, 786-0 hypoxia and 786-0 hypoxia +
Zn samples was evaluated by RT-qPCR and Western blot. Cell viability assays demonstrated that 100mM of Zn on
ccRCC cells showed a significant inhibitory effect. Gene expression analysis by RT-qPCR comparing 786-0 with and
without Zn supplementation, under normoxia and hypoxia conditions, showed decreased levels of HIF-1a - 73.75 ±
20.32% (P<0.001 vs. 786-0); 67.99 ± 4.26% (P<0.001 vs. 786-0 hypoxia) and VEGF-a - 60.72 ± 17.58% (P<0.05 vs.
786-0); 56.62 ± 8.07% (P<0.05 vs. 786-0 hypoxia). Western Blot data corroborated the RT-qPCR results. Zinc Chloride
demonstrates an antiangiogenic potential in ccRCC cells. Additional experiments are going to be done to
comprehensively understand the pathophysiological mechanisms of Zinc Chloride as a novel treatment for ccRCC.
Como referenciar
CARLOS, G.O.; NETO, B.M.; FERREIRA, L.S.; TEIXEIRA, L.F.S.; BELLINI, M.H. Evaluation of the antiangiogenic activity of zinc in renal adenocarcinoma in a hypoxic microenvironment. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 53rd, May 18-21, 2024, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2024. p. 337-337. Disponível em: https://repositorio.ipen.br/handle/123456789/49118. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.