Determination of Chinese hamster ovary cell-derived recombinant thyrotropin by reversed-phase liquid chromatography
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Journal of Chromatography, B
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A reversed-phase high-performance liquid chromatography (RP-HPLC) methodology for the qualitative and quantitative analysis of human thyrotropin (hTSH) in CHO cell conditioned medium and in purified preparations has been set up and validated for accuracy, precision and sensitivity. A recovery test indicated a bias of less than 2% and intra-day and inter-day quantitative determinations presented relative standard deviations (RSD) always <7%, while sensitivity was 0.2 μg (RSD=5.6%). The novel methodology was applied to the study of the best cultivation conditions and was able to detect a significant difference in retention time (tR) between pituitary and recombinant hTSH, probably reflecting the influence of the heterogeneity of the carbohydrate moiety on the hydrophobic properties of the molecule.
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OLIVEIRA, J.E.; MENDONCA, F.; PERONI, C.N.; BARTOLINI, P.; RIBELA, M.T.C.P. Determination of Chinese hamster ovary cell-derived recombinant thyrotropin by reversed-phase liquid chromatography. Journal of Chromatography, B, v. 787, n. 2, p. 345-355, 2003. DOI: 10.1016/S1570-0232(02)00965-0. Disponível em: http://repositorio.ipen.br/handle/123456789/5889. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.