Proteomic analysis of soluble proteins retrieved from Duttaphrynus melanostictus skin secretion by IEx-batch sample preparation
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Journal of Proteomics
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Amphibians display a toxic secretion that works as chemical defenses against predators and/or microorganisms
that is stored in specialized glands located in the tegument. For some animals, such glands have accumulated in
specific regions of the body and formed prominent structures known as macroglands. The Bufonidae family
displays conspicuous macroglands in a post-orbital position, termed parotoids, which secretions are known to be
extremely viscous and rich in alkaloids and steroids. Few proteins have been described in this material, though.
Mainly, because of the difficulties to handle such biological matrix. In this context, we have performed a proteomic
study on the parotoid macrogland secretion of the Asian bufonid Duttaphrynus melanostictus. By employing
the Ion-Exchange (IEx)-batch chromatography (anionic, cationic and both) we obtained six fractions -
bound and unbound – that were submitted to an in solution-trypsin digestion followed by LC-MS/MS. Proteins
related to: antioxidant activity, binding processes (carbohydrate/lipid/protein), energy metabolism, hydrolases,
lipid metabolism and membrane traffic were identified. Moreover, IEx was able to preserve the biological activity
of the retrieved proteins (peptidasic). The current study increases the knowledge on the proteins present in
the bufonids parotoid macrogland secretion, providing a better understanding of the physiological role played by
such molecules.
Significance: The current approach allowed a detailed proteomic analysis of the several proteins synthesized in
the D. melanostictus parotoid macrogland (Bufonidae) that are secreted into the skins, but embedded within a
complex viscous biological matrix. Moreover, our results aim to increase the knowledge on the biological role
played by such proteins at the skin.
Como referenciar
MARIANO, DOUGLAS O.C.; PREZOTTO-NETO, JOSE P.; SPENCER, PATRICK J.; SCIANI, JULIANA M.; PIMENTA, DANIEL C. Proteomic analysis of soluble proteins retrieved from Duttaphrynus melanostictus skin secretion by IEx-batch sample preparation. Journal of Proteomics, v. 209, p. 1-24, 2019. DOI: 10.1016/j.jprot.2019.103525. Disponível em: http://repositorio.ipen.br/handle/123456789/30471. Acesso em: 11 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.