Monitoring the progress and healing status of burn wounds using infrared spectroscopy
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Applied Spectroscopy
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Burns are one of the leading causes of morbidity worldwide and the most costly traumatic injuries. A better understanding
of the molecular mechanisms in wound healing is required to accelerate tissue recovery and reduce the health economic
impact. However, the standard techniques used to evaluate the biological events associated to wound repair are laborious,
time-consuming, and/or require multiple assays/staining. Therefore, this study aims to evaluate the feasibility of Fourier
transform infrared (FT-IR) spectroscopy to monitor the progress and healing status of burn wounds. Burn injuries were
induced on Wistar rats by water vapor exposure and biopsied for further histopathological and spectroscopic evaluation at
four time-points (3, 7, 14, and 21 days). Spectral data were preprocessed and compared by principal component analysis.
Pairwise comparison of post-burn groups to each other revealed that metabolic activity induced by thermal injury
decreases as the healing progresses. Higher amounts of carbohydrates, proteins, lipids, and nucleic acids were evidenced
on days 3 and 7 compared to healthy skin and reduced amounts of these molecular structural units on days 14 and 21 postburn.
FT-IR spectroscopy was used to determine the healing status of a wound based on the biochemical information
retained by spectral signatures in each phase of healing. Our findings demonstrate that FT-IR spectroscopy can monitor the
biological events triggered by burn trauma as well as to detect the wound status including full recovery based on the
spectral changes associated to the biochemical events in each phase.
Como referenciar
CASTRO, PEDRO A.A.; LIMA, CASSIO A.; MORAIS, MYCHEL R.P.T.; ZORN, TELMA M.T.; ZEZELL, DENISE M. Monitoring the progress and healing status of burn wounds using infrared spectroscopy. Applied Spectroscopy, v. 74, n. 7, p. 758-766, 2020. DOI: 10.1177/0003702820919446. Disponível em: http://repositorio.ipen.br/handle/123456789/31346. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.