The effect of radiation dose rate over the formation of protein-based nanoparticles for nanosized delivery of chemo and radiotherapeutics
Carregando...
Data
Data de publicação:
2018
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
É parte de
CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 23.
Exportar
Resumo
Recent studies demonstrated the development of papain and bovine serum albumin
nanoparticles using gamma radiation (10 kGy) in presence of 20-30% (v/v) ethanol.
With the purpose of producing stable and well defined nanocarriers, this work aims to
determine the influence of different dose rates over protein nanoparticle formation. For
this purpose, papain and BSA nanoparticles were synthetized in phosphate buffer (50
mM, pH 7.2) and ethanol (20-30%, v/v) using a radiation dose of 10 kGy and dose rate
of 0.8, 2, 5 and 10 kGy.h-1. After irradiation, samples were evaluated by dynamic light
scattering, fluorescence and proteolytic activity to verify the size, secondary structure
and monitoring of the enzymatic activity, respectively. For papain nanoparticles it was
observed that the dose rate did not influence the particle size formation, however
crosslinking evidenced by bityrosine showed that samples irradiated at 0.8 and 5
kGy.h-1 presented higher bityrosine levels. On the other hand, BSA nanoparticles
presented different results if compared to papain NPs. Different dose rates caused
different and non-linear size increase for each condition, following the order: 5 > 10 >
0.8 > 2 kGy.h-1. However, in terms of crosslinking formation, a linear increase was
registered, as at 0.8 kGy.h-1 the smallest signal was achieved, whereas at 10 kGy.h-
1 the highest signal was recorded. In conclusion, BSA nanoparticles were more
sensitive to different radiation dose rates than nanopapain. Optimized results in terms
of size increase and higher bityrosine levels were observed for the samples irradiated
at 5 kGy.h-1, in which nanoparticle formation will occur faster if compared to the
synthesis carried out under distinct conditions. As final applications of the system
concert their use for the delivery of chemo or radiotherapeutics, the loading of
paclitaxel, a well-known chemotherapeutic agent, and radiolabeling with tecntetium-
99m, a radioisotope suitable for biomedical applications, have also been performed
with high efficiency, thus demonstrating a proof of concept of such systems.
Como referenciar
VARCA, G.H.C.; FAZOLIN, G.N.; FERREIRA, A.H.; OLIVEIRA, J.P.R. de; MARQUES, F.; LUGAO, A.B. The effect of radiation dose rate over the formation of protein-based nanoparticles for nanosized delivery of chemo and radiotherapeutics. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 23., 04-08 de novembro, 2018, Foz do Iguaçu, PR. Resumo... p. 8875-8875. Disponível em: http://repositorio.ipen.br/handle/123456789/29677. Acesso em: 15 Jun 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.