Evaluation of the detoxification mechanisms of metals in aquatic organisms by characterization of hepatic metallothionein (MT)
Carregando...
Data
Data de publicação
2014
Autores IPEN
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
É parte de
ANNUAL MEETING OF THE SOCIETY OF ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 24th
Exportar
Resumo
Metallothionein (MT) has been thoroughly used as a biomarker of metals exposure.
MT is a low-molecular-weight protein which has many sulfhydryl groups due to the
large amount of cysteine in the molecule. These sulfhydryl groups bind a variety of
metals and therefore, presumably, make them less toxic to other cellular
constituents. However, biochemistry aspects of the protein as isoforms
identification and quantification and their specific mechanisms of detoxification in
sentinel species are, as yet, weak. Previous data showed that fish, such as the catfish
Cathrorops spixil, are efficient bioindicator species to metals contamination in
coastal aquatic areas under anthropogenic influence, for instance inputs of Pb, Cd,
Hg, Ni, Fe, Zn, Cu and Mn. C. spixil is the most common catfish in the Brazilian
coast and has a feeding habit mainly of materials and organisms upon the sediment,
where the availability of contaminants is high. In order to understand intrinsic
aspects of the detoxification process of toxic metals in sentinel species in natural
and anthropogenic conditions, hepatic samples of C. spixii were collected in a
non-polluted (Cananeia), as well as one polluted, estuary (Santos-São Vicente) and
tested under different assays in order to establish an effective bioanalytical
technique to purify the protein like-MT, identify and quantify the specific isoforms
and the metal contents in the cell. Therefore, hepatic samples were submitted to
ultracentrifugation, thermocoagulation and a chromatographic purification and
identification of MT isoforms by size-exclusion-HPLC and anion-exchange-HPLC,
respectively. The elution of the protein was made with online UV/Vis detection.
Metals in hepatic cytosols were also quantified by HR-ICP-MS. Obtained results
showed that the established protocol of bioanalytical technique was effective to
purify MT-like protein by SE-HPLC and to identify its isoforms by AE-HPLC.
With these analytical strategies, it was possible to identify two kinds of MT
isoforms (MT-1 and MT-2) in the C. spixil. Fish MT-1 from polluted areas showed
a strong linkage with the levels of Cu, Hg, Pb, Fe and Ni suggesting that the MT-1 is
an effective biomarker of metal contamination.
Como referenciar
AZEVEDO, J.D.; SARKIS, J.E.; ROGERO, S.O. Evaluation of the detoxification mechanisms of metals in aquatic organisms by characterization of hepatic metallothionein (MT). In: ANNUAL MEETING OF THE SOCIETY OF ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 24th, May 11-15, 2014, Basel, Switzerland. Abstract... p. 151-151. Disponível em: http://repositorio.ipen.br/handle/123456789/27655. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.