Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes
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Cell and Tissue Banking
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The possibility of obtaining transplantable
oral epithelia opens new perspectives for oral treatments. Most of them are surgical, resulting in mucosal
failures. As reconstructive material this in vitro
epithelia would be also useful for other parts of the
human body. Many researchers still use controversial
methods; therefore it was evaluated and compared the
efficiency of the enzymatic and direct explant methods to obtain oral keratinocytes. To this project oral
epithelia fragments were used. This work compared:
time needed for cell obtainment, best cell amount,
life-span and epithelia forming cell capacity. The
results showed the possibility to obtain keratinocytes
from a small oral fragment and we could verify the
advantages and peculiar restrictions. We concluded
that under our conditions the enzymatic method
showed the best results: in the cells obtaining time
needed, cell amount and life-span. Both methods
showed the same capacity to form in vitro epithelia.
Como referenciar
KLINGBEIL, MARIA F.G.; HERSON, MARISA R.; CRISTO, ELIER B.; PINTO JUNIOR, DECIO dos S.; YOSHITO, DANIELE; MATHOR, MONICA B. Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes. Cell and Tissue Banking, v. 10, n. 3, p. 197-204, 2009. DOI: 10.1007/s10561-009-9122-7. Disponível em: http://repositorio.ipen.br/handle/123456789/4838. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.