S-phase reduction in T47D human beast cancer epithelial cells induced by an S100P antisense-retroviral construct
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Oncology Reports
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S100P is expressed in several malignant neoplasms.
It was previously demonstrated that S100P is involved in the
very early stages of breast carcinogenesis. In the present
study we used a retrovirus-mediated transfer of antisenseS100P in order to check whether the decrease in expression
of this protein could lead to alterations in the cell cycle of
epithelial cells of human breast cancer. The T47D breast
carcinoma cell line, a human breast epithelial cell that
expresses high levels of S100P, was a tool used in this study
to investigate the alteration in cell cycle induced by a
retrovirus-mediated transfer of antisense-S100P. First we
used the real-time PCR technique to quantify the gene
expression. The results showed a reduction of 63% of
expression within the T47D-S100P-A/S infected population
compared with control T47D-LXSN clones. To determine
the impact of the S100P antisense technique on protein
expression in T47D cells, we performed immunofluorescence
staining and analyzed the resulting images using a confocal
microscope. The images showed much less pronounced
antibody marking of the S100P protein in the T47D-S100PA/S compared with control cells. To evaluate whether the
antisense approach caused any alteration in the cell cycle, we
concluded the study with flow cytometric analysis of the cell
distribution. Our findings indicated that, in our model,
S100P-antisense cells showed a 23% reduction of cells at the
S-phase. Using transduction techniques with an S100P
antisense-retroviral construct we were able to demonstrate a
significant reduction in S-phase of the T47D cell cycle. To
the best of our knowledge, this is the first time that an
antisense approach has been used against S100P mRNA in
breast cancer epithelial cells. The results showed here seem
to further classify S100P as a protein that might be involved
in the cell cycle imbalance observed during breast carcinogenesis.
Como referenciar
BEISSEL, BETTINA; SILVA, ISMAEL D.C.G.; PESQUERO, JOÃO B.; RUSSO, JOSE; SCHOR, NESTOR; BELLINI, MARIA H. S-phase reduction in T47D human beast cancer epithelial cells induced by an S100P antisense-retroviral construct. Oncology Reports, v. 17, p. 611-615, 2007. DOI: 10.3892/or.17.3.611. Disponível em: http://repositorio.ipen.br/handle/123456789/5200. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.