A simple and quick method to generate in vitro tridimensional tumor bodies from a human breast adenocarcinoma (MCF7) using magnetic aggregation technique
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Biophysical Reviews
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INTRODUCTION
Tumor physiology studies have to rely on efficient and representative
models, as animal-based or in vitro tridimensional cell constructs. The
work used magnetite (Fe3O4) nanoparticles produced by electron-beam
induced chemical reduction to give cells the ability to form aggregates
when submitted to a magnetic field, and thus to produce micro tumors
in vitro.
OBJECTIVES
The work aimed to produce human breast adenocarcinoma mini tumors
(BAMT's) in vitro.
MATERIALS AND METHODS
Paramagnetic iron oxide nanoparticles (PION’s) were synthesized
through electron-beam induced Fe3+ reduction and subsequent
coprecipitation. Due to its poly-L-lysine coating, PION’s were adsorbed
on cell membranes ofMCF7 (human breast adenocarcinoma). Cells were
seeded in 24-well cell culture plates pre-treated overnight with Pluronic®
F-127 to prevent cell adhesion and kept in culture conditions under magnetic
fields for at least 6 days. BAMT's were differentially stained with
Hoescht 33342 and ethidium bromide and imaged by wide-field fluorescence
microscopy.
DISCUSSION AND RESULTS
BAMT's appeared as integer and well-defined cellular aggregates, with
sparse dead cells stained by ethidium bromide. These structures can be
further used for in vitro tumor studies, as BAMT’s are supposed to be
more reliable models than monolayer cultures. Treatment of wells with
poloxamer caused a mild to moderated cell-repellent effect, similar to
those found in commercially available products, only by a fraction of
the cost.
CONCLUSION
The experiments succesfully produced mini tumors prone to be used in
in vitro studies.
Como referenciar
LIMA, MAYELLE M.P.; NASCIMENTO, PAMELA F. do; NASCIMENTO, ANA C.G.; VIEIRA, DANIEL P. A simple and quick method to generate in vitro tridimensional tumor bodies from a human breast adenocarcinoma (MCF7) using magnetic aggregation technique. Biophysical Reviews, v. 13, n. 6, p. 1470-1470, 2021. DOI: 10.1007/s12551-021-00845-2. Disponível em: http://repositorio.ipen.br/handle/123456789/32893. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.