Influence of the reference spectrum used as input to the RMieS-EMSC algorithm for correction of spectral distortions induced by Mie scattering in FTIR hyperspectral images
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2019
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SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE
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Fourier transform infrared (FTIR) microspectroscopy has shown great promise as a
tool to assess the histological architecture of tissue samples by providing morphochemical maps that enable the evaluation of both spatial and compositional information based on
the signatures of molecular vibrations. However, FTIR datasets collected in transmission
and transflection modes are subjected to undesired spectral contributions non-related
to the phenomenon of light absorption that must be corrected prior any analysis. The
algorithm so-called “Resonant Mie Scattering – Extended Multiplicative Scatter Correction
(RMieS-EMSC)” has been well succeeded in vibrational spectroscopy in removing spectral
distortions. The main idea of the algorithm is to reconstruct the datasets that present
undesired spectral contributions based on a scatter-free reference spectrum. In this study,
FTIR hyperspectral images acquired from cutaneous malignant lesions were submitted
to RMieS-EMSC protocol using Matrigel and average spectrum in order to evaluate the
influence of the reference spectrum in the final correction. False-color maps obtained after
segmentation were compared in terms of image quality and consistency with standard
histopathology in order to evaluate the ability of each method to reproduce the histological
structures of the specimen. Our findings indicate that the choice of reference spectrum
has very little difference on the outcome of image analysis. The images obtained by both
spectra used as reference may not have resulted in identical false-color maps, but the
differences between the morphochemical maps are relatively small.
Como referenciar
LIMA, CASSIO; CORREA, LUCIANA; BYRNE, HUGH; ZEZELL, DENISE M. Influence of the reference spectrum used as input to the RMieS-EMSC algorithm for correction of spectral distortions induced by Mie scattering in FTIR hyperspectral images. In: SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE, October 7-9, 2019, São Paulo, SP. Abstract... Piscataway, NJ, USA: IEEE, 2019. p. 47-48. Disponível em: http://repositorio.ipen.br/handle/123456789/32227. Acesso em: 30 Dec 2025.
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