Co-cultivation of mesenchymal stem cells and skin keratinocytes on electrospun scaffolds
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2014
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TERMIS-AM CONFERENCE
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Available treatments in skin regeneration are insufficient for
promoting healing. This study has aimed to produce a cutaneous
substitute combining mesenchymal stem cells (MSCs), keratinocytes
and a PDLLA biomaterial, constructed by electrospinning, into 3
groups: 1) PDLLA, 2) PDLLA/NaOH, scaffolds with hydrolyzed
surfaces and 3) PDLLA/Lam, hydrolyzed and with laminin binding.
The MSCs were seeded at the bottom of the scaffolds. After 24 hours,
skin keratinocytes were seeded at the top. The scaffolds were characterized
by morphology, fiber diameter, cell adhesion on the day of
the seeding and viability on days 7, 14 and 21. As a result, the
scaffolds presented randomly distributed, well formed fibers. The
fiber diameter for all the groups was 4.58 mmfor the largest fibers and
574 nm for the smallest. The pore sizes of the scaffolds obtained were
approximately 27.5 mm and 3.44 mm, for the largest and smallest fibers, respectively. In biological analysis, cell adhesion was greater
in the PDLLA/Lam scaffolds with absorbance of 2.268 – 0.494, in
comparison with 1.264 – 0.473 for the PDLLA and 1.159 – 0.120
for the PDLLA/NaOH scaffolds. There was an improvement in the
number of viable cells from day 7 to day 21 of analysis. In general,
the PDLLA/Lam group showed the best results for cell adhesion and
viability tests. In conclusion, the PDLLA scaffolds, mainly the
PDLLA/Lam groups, showed good results for the co-cultivation of
the cells, with good cell adhesion and the presence of viable cells.
Therefore, these scaffolds show promise to be suitable biomaterials
for use in tissue engineering.
Como referenciar
STEFFENS, D.; SANTI, B.T.; LUCO, D.P.; MATHOR, M.B.; PRANKE, P. Co-cultivation of mesenchymal stem cells and skin keratinocytes on electrospun scaffolds. In: TERMIS-AM CONFERENCE, December 13-16, 2014, Washington, DC, USA. Abstract... p. S-89 - S-89. Disponível em: http://repositorio.ipen.br/handle/123456789/27665. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.