Physical-chemical and biological characterization of different preparations of equine chorionic gonadotropin

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dc.contributor.authorALVAREZ, RAFAEL H.
dc.contributor.authorNATAL, FABIO L.N.
dc.contributor.authorRIBELA, MARIA T.C.P.
dc.contributor.authorALMEIDA, BEATRIZ E. de
dc.contributor.authorOLIVEIRA, JOAO E. de
dc.contributor.authorBARTOLINI, PAOLO
dc.coverageInternacionalpt_BR
dc.date.accessioned2017-03-13T18:37:11Z
dc.date.available2017-03-13T18:37:11Z
dc.date.issued2016pt_BR
dc.description.abstractOvarian stimulation with commercial preparations of equine chorionic gonadotropin (eCG) produces extremely variable responses in domestic animals, ranging from excessive stimulation to practically no stimulation, when applied on the basis of their declared unitage. This study was conducted to analyze four commercial preparations from different manufacturers via reversed-phase HPLC (RP-HPLC) in comparison with a reference preparation and an official International Standard from the World Health Organization. The peaks obtained by this qualitative and quantitative physical–chemical analysis were compared using an in vivo bioassay based on the ovarian weight gain of prepubertal female rats. The RP-HPLC data showed one or two peaks close to a main peak (tR = 27.9 min), which were related to the in vivo bioactivity. Commercial preparations that have this altered peak showed very little or no in vivo activity, as demonstrated by rat ovarian weight and in peripubertal gilts induced to ovulate. Overall, these findings indicate that RP-HPLC can be a rapid and reliable tool to reveal changes in the physicochemical profile of commercial eCG that is apparently related to decreased biological activity of this hormone.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDFAPESP: 11/13096-0pt_BR
dc.format.extent459-465pt_BR
dc.identifier.citationALVAREZ, RAFAEL H.; NATAL, FABIO L.N.; RIBELA, MARIA T.C.P.; ALMEIDA, BEATRIZ E. de; OLIVEIRA, JOAO E. de; BARTOLINI, PAOLO. Physical-chemical and biological characterization of different preparations of equine chorionic gonadotropin. <b>Journal of Veterinary Science</b>, v. 17, n. 4, p. 459-465, 2016. DOI: <a href="https://dx.doi.org/10.4142/jvs.2016.17.4.459">10.4142/jvs.2016.17.4.459</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/27152.
dc.identifier.doi10.4142/jvs.2016.17.4.459pt_BR
dc.identifier.fasciculo4pt_BR
dc.identifier.issn1229-845Xpt_BR
dc.identifier.percentilfi64.34
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/27152
dc.identifier.vol17pt_BR
dc.relation.ispartofJournal of Veterinary Sciencept_BR
dc.rightsopenAccesspt_BR
dc.subjectgonadotropins
dc.subjecthigh pressure
dc.subjectliquid column chromatography
dc.subjectbioassay
dc.subjectovaries
dc.subjectanimal cells
dc.subjecthorses
dc.titlePhysical-chemical and biological characterization of different preparations of equine chorionic gonadotropinpt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorPAOLO BARTOLINI
ipen.autorJOAO EZEQUIEL DE OLIVEIRA
ipen.autorBEATRIZ ELANE DE ALMEIDA
ipen.autorMARIA TERESA DE CARVALHO PINTO RIBELA
ipen.codigoautor1503
ipen.codigoautor425
ipen.codigoautor6043
ipen.codigoautor1197
ipen.contributor.ipenauthorPAOLO BARTOLINI
ipen.contributor.ipenauthorJOAO EZEQUIEL DE OLIVEIRA
ipen.contributor.ipenauthorBEATRIZ ELANE DE ALMEIDA
ipen.contributor.ipenauthorMARIA TERESA DE CARVALHO PINTO RIBELA
ipen.date.recebimento17-03pt_BR
ipen.identifier.fi1.164pt_BR
ipen.identifier.ipendoc23500pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi0.001 - 1.499
ipen.range.percentilfi50.00 - 74.99
ipen.type.genreArtigo
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relation.isAuthorOfPublicatione9e6346c-9b8b-4d39-8bdd-505ea6997646
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relation.isAuthorOfPublication.latestForDiscovery2c1f5cfd-da46-4b49-b209-77aadfb388f1
sigepi.autor.atividadeRIBELA, MARIA T.C.P.:1197:810:Npt_BR
sigepi.autor.atividadeALMEIDA, BEATRIZ E. de:6043:-1:Npt_BR
sigepi.autor.atividadeOLIVEIRA, JOAO E. de:425:810:Npt_BR
sigepi.autor.atividadeBARTOLINI, PAOLO:1503:810:Npt_BR
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