Silencing of nuclear factor kappa b 1 gene expression inhibits colony formation, cell migration and invasion via the downregulation of interleukin 1 beta and matrix metallopeptidase 9 in renal cell carcinoma
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Molecular Biology Reports
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Renal cell carcinoma (RCC) is a highly deadly urological tumor due to its high metastatic incidence and its notorious
chemoresistance. The nuclear transcription factor kappa B (NF-κB) family has been associated with apoptosis resistance
and cellular invasion in RCC. The purpose of this study was to evaluate the impact of NF-κB1 gene silencing on the colony
formation, cell migration and invasion abilities of the RCC cell line. Renca–mock and Renca-shRNA-NF-κB1 cells were
used in this work. NF-κB1 downregulation was assessed by western blotting. The mRNA expression levels of interleukin-1
beta (IL-1β) and MMP-9 were assessed by real-time quantitative polymerase chain reaction (RT-qPCR). The IL-1β levels
in the culture media were determined by a commercial ELISA kit. The MMP-9 protein expression and gelatinolytic activity
were evaluated by western blotting and zymography, respectively, and the migration and invasion abilities were analysed.
The expression levels of p105 and p50 in Renca-shRNA-NF-κBmoc1 cells were significantly reduced compared with those
in the Renca–mock cells. The colony numbers of shRNA-NF-кB1 cells were lower than the colony numbers of the Renca–
mock cells. NF-κB1 knockdown inhibited the cell migration and invasion of Renca-shRNA-NF-κB1 cells. These cells also
exhibited reduced levels of IL-1β. The MMP-9 expression and activity levels were significantly reduced in Renca-shRNANF-
κB1 cells. Taken together, these results indicate that the downregulation of NF-κB1 suppresses the tumourigenicity of
RCC by reducing MMP-9 expression and activity; thus, NF-κB1 could be a molecular target for RCC treatment.
Como referenciar
TEIXEIRA, LUIZ F.S.; PERON, JEAN P.S.; BELLINI, MARIA H. Silencing of nuclear factor kappa b 1 gene expression inhibits colony formation, cell migration and invasion via the downregulation of interleukin 1 beta and matrix metallopeptidase 9 in renal cell carcinoma. Molecular Biology Reports, v. 47, n. 2, p. 1143-1151, 2020. DOI: 10.1007/s11033-019-05212-9. Disponível em: http://repositorio.ipen.br/handle/123456789/31107. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.