Biophysical properties of electrospun chitosan-grafted poly(lactic acid) nanofibrous scaffolds loaded with chondroitin sulfate and silver nanoparticles

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dc.contributor.authorJUNIOR, ALEXANDRE F.pt_BR
dc.contributor.authorRIBEIRO, CHARLENE A.pt_BR
dc.contributor.authorLEYVA, MARIA E.pt_BR
dc.contributor.authorMARQUES, PAULO S.pt_BR
dc.contributor.authorSOARES, CARLOS R.J.pt_BR
dc.contributor.authorQUEIROZ, ALVARO A.A. dept_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2022-01-27T15:44:12Z
dc.date.available2022-01-27T15:44:12Z
dc.date.issued2022pt_BR
dc.description.abstractThe aim of this work was to study the biophysical properties of the chitosan-grafted poly(lactic acid) (CH-g-PLA) nanofibers loaded with silver nanoparticles (AgNPs) and chondroitin-4-sulfate (C4S). The electrospun CH-g-PLA:AgNP:C4S nanofibers were manufactured using the electrospinning technique. The microstructure of the CH-g-PLA:AgNP:C4S nanofibers was investigated by proton nuclear magnetic resonance (1H-NMR), scanning electron microscopy (SEM), UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD), and Fourier transform infrared (ATR-FTIR) spectroscopy. ATR-FTIR and 1H-NMR confirm the CH grafting successfully by PLA with a substitution degree of 33.4%. The SEM measurement results indicated apparently smooth nanofibers having a diameter range of 340 ± 18 nm with porosity of 89 ± 3.08% and an average pore area of 0.27 μm2. UV-Vis and XRD suggest that silver nanoparticles with the size distribution of 30 nm were successfully incorporated into the electrospun nanofibers. The water contact angle of 12.8 ± 2.7° reveals the hydrophilic nature of the CH-g-PLA:AgNP:C4S nanofibers has been improved by C4S. The electrospun CH-g-PLA:AgNP:C4S nanofibers are found to release ions Ag+ at a concentration level capable of rendering an antimicrobial efficacy. Gram-positive bacteria (S.aureus) were more sensitive to CH-g-PLA:AgNP:C4S than Gram-negative bacteria (E. coli). The electrospun CH-g-PLA:AgNP:C4S nanofibers exhibited no cytotoxicity to the L-929 fibroblast cells, suggesting cytocompatibility. Fluorescence microscopy demonstrated that C4S promotes the adhesion and proliferation of fibroblast cells onto electrospun CH-g-PLA:AgNP:C4S nanofibers.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipIDCNPq: 307609/2018-9pt_BR
dc.format.extent1098-1110pt_BR
dc.identifier.citationJUNIOR, ALEXANDRE F.; RIBEIRO, CHARLENE A.; LEYVA, MARIA E.; MARQUES, PAULO S.; SOARES, CARLOS R.J.; QUEIROZ, ALVARO A.A. de. Biophysical properties of electrospun chitosan-grafted poly(lactic acid) nanofibrous scaffolds loaded with chondroitin sulfate and silver nanoparticles. <b>Journal of Biomaterials Applications</b>, v. 36, n. 6, p. 1098-1110, 2022. DOI: <a href="https://dx.doi.org/10.1177/08853282211046418">10.1177/08853282211046418</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/32667.
dc.identifier.doi10.1177/08853282211046418pt_BR
dc.identifier.fasciculo6pt_BR
dc.identifier.issn0885-3282pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7982-1789
dc.identifier.percentilfi28.5pt_BR
dc.identifier.percentilfiCiteScore52pt_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/32667
dc.identifier.vol36pt_BR
dc.relation.ispartofJournal of Biomaterials Applicationspt_BR
dc.rightsopenAccesspt_BR
dc.subjectsilver
dc.subjectnanoparticles
dc.subjectnanofibers
dc.subjectlactic acid
dc.titleBiophysical properties of electrospun chitosan-grafted poly(lactic acid) nanofibrous scaffolds loaded with chondroitin sulfate and silver nanoparticlespt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorÁLVARO ANTONIO ALENCAR DE QUEIROZ
ipen.autorCARLOS ROBERTO JORGE SOARES
ipen.codigoautor7319
ipen.codigoautor509
ipen.contributor.ipenauthorÁLVARO ANTONIO ALENCAR DE QUEIROZ
ipen.contributor.ipenauthorCARLOS ROBERTO JORGE SOARES
ipen.date.recebimento22-01
ipen.identifier.fi2.9pt_BR
ipen.identifier.fiCiteScore4.8pt_BR
ipen.identifier.ipendoc28435pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi1.500 - 2.999
ipen.range.percentilfi25.00 - 49.99
ipen.type.genreArtigo
relation.isAuthorOfPublicatione7d5bfcd-02bd-4e2a-8b3a-b4a56eb4cdfa
relation.isAuthorOfPublicationd6719ab2-f2e9-4d7c-9cea-a6316fc14c9e
relation.isAuthorOfPublication.latestForDiscoveryd6719ab2-f2e9-4d7c-9cea-a6316fc14c9e
sigepi.autor.atividadeQUEIROZ, ALVARO A.A. de:7319:810:Npt_BR
sigepi.autor.atividadeSOARES, CARLOS R.J.:509:810:Npt_BR
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