Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros
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Journal of Venomous Animals and Toxins including Tropical Diseases
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Background: Venoms represent a still underexplored reservoir of bioactive components that might mitigate or
cure diseases in conditions in which conventional therapy is ineffective. The bradykinin-potentiating peptides (BPPs)
comprise a class of angiotensin-I converting enzyme (ACE) inhibitors. The BPPs usually consist of oligopeptides with
5 to 13 residues with a high number of proline residues and the tripeptide Ile-Pro-Pro (IPP-tripeptide) in the C-terminus
region and have a conserved N-terminal pyroglutamate residue. As a whole, the action of the BPPs on prey and
snakebite victims results in the decrease of the blood pressure. The aim of this work was to isolate and characterize
novel BPPs from the venom of Bitis gabonica rhinoceros.
Methods: The crude venom of B. g. rhinoceros was fractionated by size exclusion chromatography and the peptide
fraction (<7 kDa) was separated by reverse phase chromatography (RP-HPLC) and analyzed by ESI-IT-TOF-MS/MS. One
new BPP was identified, synthetized and assayed for ACE inhibition and, in vivo, for edema potentiation.
Results: Typical BPP signatures were identified in three RP-HPLC fractions. CID fragmentation presented the usual
y-ion of the terminal P-P fragment as a predominant signal at m/z 213.1. De novo peptide sequencing identified
one Bothrops-like BPP and one new BPP sequence. The new BPP was synthesized and showed poor inhibition
over ACE, but displayed significant bradykinin-induced edema potentiation.
Conclusions: So far, few BPPs are described in Viperinae, and based on the sequenced peptides, two non-canonical
sequences were detected. The possible clinical role of this new peptides remains unclear.
Como referenciar
FUCASE, TAMARA M.; SCIANI, JULIANA M.; CAVALCANTE, INGRID; VIALA, VINCENT L.; CHAGAS, BRUNO B.; PIMENTA, DANIEL C.; SPENCER, PATRICK J. Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, n. 33, p. 1-8, 2017. DOI: 10.1186/s40409-017-0124-9. Disponível em: http://repositorio.ipen.br/handle/123456789/27741. Acesso em: 26 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.