Hybrid gold-protein nanoparticles as radiosensitizers

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dc.contributor.authorFREITAS, L.F.pt_BR
dc.contributor.authorCRUZ, C.C. dapt_BR
dc.contributor.authorBATISTA, J.G.pt_BR
dc.contributor.authorVARCA, G.H.pt_BR
dc.contributor.authorLUGAO, A.B.pt_BR
dc.contributor.authorMATHOR, M.B.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoPAN-AMERICAN NANOTECHNOLOGY CONFERENCE, 2ndpt_BR
dc.date.accessioned2021-03-08T19:05:07Z
dc.date.available2021-03-08T19:05:07Z
dc.date.eventoMarch 4-7, 2020pt_BR
dc.description.abstractGold nanoparticles present unique optical properties which are dependent upon size and morphology, and consist on a differential interaction with radiation compared to the bulk material. Those nanoparticles can be modified in order to adjust their bioavailability and tissue-targeting, and one of the means to do so is by adsorbing one or more types of proteins onto their surface. Gamma radiation can be helpful in this regard, since it promotes intra- and intermolecular crosslinks in proteins and enables their adsorption onto the metallic nanoparticles’ surfaces. Here we present the results obtained for hybrid gold-protein nanoparticles as radiosensitizers. The nanoparticles were synthesized radiolytically by mixing 5 mmol L-1 NaAuCl4 with 1 mg mL-1 bovine serum albumin (BSA) or papain in the presence of 0.1 mol L-1 tert-butanol and 20% ethanol. The solutions were irradiated with 10 kGy in a multipurpose gamma irradiator (60Co source, 5 kGy per hour) for the radiolytic synthesis of the nanoparticles, and then the resulting red suspension was stored until use. 10^4 cells (MDA-MB-231 line) were seeded in 96-well plates and incubated with a 2:1 mixture of DMEM medium and nanoparticles suspension for 12 hours. Then, the wells were washed with sterile phosphate buffered saline, and fresh DMEM medium was added prior to irradiation in a gamma cell (60Co source, 0.6 kGy per hour) with 10, 30 and 50 Gy. 48 hours later, the cell viability was assessed by MTS assay. The results indicate that the radiation alone slightly stimulated the proliferation of the tumor cells, but this effect was more evident in the presence of gold-papain nanoparticles. The ablative effect due to radiosensitization was observed with 30 and 50 Gy for the cells incubated with gold-BSA nanoparticles, and 10 and 30 Gy for the cells incubated with gold-papain nanoparticles. This difference might be due to a more effective internalization or surface-attachment of nanoparticles when they are coated with papain, and one evidence for this assumption is the fact that the cell culture becomes red after the incubation with gold-papain nanoparticles. Therefore, protein-coated nanoparticles might be effective as radiosensitizers, depending on the coating and dose of radiation.pt_BR
dc.event.siglaPANNANOpt_BR
dc.identifier.citationFREITAS, L.F.; CRUZ, C.C. da; BATISTA, J.G.; VARCA, G.H.; LUGAO, A.B.; MATHOR, M.B. Hybrid gold-protein nanoparticles as radiosensitizers. In: PAN-AMERICAN NANOTECHNOLOGY CONFERENCE, 2nd, March 4-7, 2020, Águas de Lindoia, SP. <b>Abstract...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/31906.
dc.identifier.orcid0000-0002-7294-9106pt_BR
dc.identifier.orcid0000-0002-1737-3191pt_BR
dc.identifier.orcid0000-0002-6545-237Xpt_BR
dc.identifier.orcidhttp://orcid.org/0000-0002-7294-9106
dc.identifier.orcidhttps://orcid.org/0000-0002-1737-3191
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/31906
dc.local.eventoÁguas de Lindoia, SPpt_BR
dc.rightsopenAccesspt_BR
dc.titleHybrid gold-protein nanoparticles as radiosensitizerspt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorJORGE GABRIEL DOS SANTOS BATISTA
ipen.autorMONICA BEATRIZ MATHOR
ipen.autorADEMAR BENEVOLO LUGAO
ipen.autorGUSTAVO HENRIQUE COSTAVARCA
ipen.autorCASSIA PRISCILA CUNHA DA CRUZ
ipen.autorLUCAS FREITAS DE FREITAS
ipen.codigoautor10274
ipen.codigoautor209
ipen.codigoautor339
ipen.codigoautor8661
ipen.codigoautor15070
ipen.codigoautor14818
ipen.contributor.ipenauthorJORGE GABRIEL DOS SANTOS BATISTA
ipen.contributor.ipenauthorMONICA BEATRIZ MATHOR
ipen.contributor.ipenauthorADEMAR BENEVOLO LUGAO
ipen.contributor.ipenauthorGUSTAVO HENRIQUE COSTAVARCA
ipen.contributor.ipenauthorCASSIA PRISCILA CUNHA DA CRUZ
ipen.contributor.ipenauthorLUCAS FREITAS DE FREITAS
ipen.date.recebimento21-03
ipen.event.datapadronizada2020pt_BR
ipen.identifier.ipendoc27677pt_BR
ipen.notas.internasAbstractpt_BR
ipen.type.genreResumo
relation.isAuthorOfPublicationd71d578f-dbbd-4ff9-adf8-929b13d23037
relation.isAuthorOfPublication742b424f-9dfb-4e4a-993b-000052bb1313
relation.isAuthorOfPublication99ac24c5-2ae1-465a-a6f2-40b4d9af6af7
relation.isAuthorOfPublication46f998cf-b207-4854-94c0-dd2def4eec2a
relation.isAuthorOfPublication296b0b7e-28f4-4658-b7d4-f52e0494fe8f
relation.isAuthorOfPublication05be75ae-89a2-47f4-a13a-7723b50a6bd0
relation.isAuthorOfPublication.latestForDiscovery05be75ae-89a2-47f4-a13a-7723b50a6bd0
sigepi.autor.atividadeMATHOR, M.B.:209:220:Npt_BR
sigepi.autor.atividadeLUGAO, A.B.:339:740:Npt_BR
sigepi.autor.atividadeVARCA, G.H.:8661:740:Npt_BR
sigepi.autor.atividadeBATISTA, J.G.:10274:740:Npt_BR
sigepi.autor.atividadeFREITAS, L.F.:14818:740:Spt_BR
sigepi.autor.atividadeCRUZ, C.C. da:15070:740:N
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