In vitro tests of resveratrol radiomodifying effect on rhabdomyosarcoma cells by comet assay
Carregando...
Data
Data de publicação
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
Toxicology in Vitro
ODS
Exportar
Resumo
Cancer is a global public health problem. Resveratrol is a defensive polyphenol that is synthesized by a
wide variety of plants in response to exposure to ultraviolet radiation or also due to mechanical stress
caused by the action of pathogens and chemical and physical agents. Grape vines have a high capacity
to produce resveratrol, so grape juice and wine, mainly red wine, are considered good sources of resveratrol. The protective effects of resveratrol include promotion of antiinflammatory response, antitumor
activity and prevention of degenerative diseases, reduced incidence of cardiovascular diseases and inhibition of platelet aggregation, among others. Therefore, resveratrol is considered to be a cell protector.
However, at high concentrations, resveratrol promotes contrary effects by sensitizing cells. The aim of
this study was to investigate in vitro the radiomodifying effect of resveratrol in culture of human rhabdomyosarcoma cells (RD) by applying the comet assay to evaluate the cell damage and repair capacity.
The LD50 (lethal dose) obtained was 499.95 ± 9.83 Gy (Mean ± SD) and the CI50 (cytotoxicity index) was
150 lM in the RD cells. Based on these data, it was defined the gamma radiation doses (50 and
100 Gy) and resveratrol concentrations (15, 30 and 60 lM) to be used in this study. The results indicated
that resveratrol acts as a cell protector at a concentration of 15 lM and has a cytotoxic effect at 60 lM.
However, with the interaction of the gamma radiation, the concentration of 60 lM did not produce a statistically significant radiosensitizing effect.
Como referenciar
MAGALHAES, V.D.; ROGERO, S.O.; CRUZ, A.S.; VIEIRA, D.P.; OKAZAKI, K.; ROGERO, J.R. In vitro tests of resveratrol radiomodifying effect on rhabdomyosarcoma cells by comet assay. Toxicology in Vitro, v. 28, n. 8, p. 1436-1442, 2014. DOI: 10.1016/j.tiv.2014.07.006. Disponível em: http://repositorio.ipen.br/handle/123456789/22928. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.