Structural characterization and enzymatic activity of the recombinant Ala959 to Ser1066 region of human ace
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Jacobs Journal of Enzymology and Enzyme Engineering
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Angiotensin-converting enzyme catalyzes the conversion of angiotensin I to the vasoconstrictor angiotensin II and the hydrolysis
of bradykinin (BK). Human somatic angiotensin-converting enzyme has two homologous domains (N and C) that share 60%
identity. Although these two regions have high homology, the catalytic site of the C-domain exhibits three-fold greater activity
than the N-domain in the hydrolysis of angiotensin I in vivo. The present study aimed to obtain the Ala959 to Ser1066 catalytic region
of the C-domain of angiotensin-converting enzyme in a structural conformation that resembles its native form. We amplified the
324-bp sequence corresponding to the catalytic site of C-domain of angiotensin-converting enzyme and cloned this sequence
into a pET28 vector. The catalytic site of C-domain of angiotensin-converting enzyme peptide was expressed in a bacterial system,
and its purification was performed in one step using a His-tag affinity column. Structural analysis by circular dichroism and
fluorescence confirmed that the purified protein is correctly folded, and catalytic site of C-domain of angiotensin-converting
enzyme possesses enzymatic activity and is inhibited by lisinopril. This peptide can be used to test new inhibitors and C-domain
of angiotensin-converting enzyme substrates because this peptide is easy to produce and this has proven efficient link with these
molecules.
Como referenciar
ELIASA, CAROLINE C.; PEREIRA, LARISSA M.; ARAGAO, DANIELLE S.; CASARINI, DULCE E.; AFFONSO, REGINA. Structural characterization and enzymatic activity of the recombinant Ala959 to Ser1066 region of human ace. Jacobs Journal of Enzymology and Enzyme Engineering, v. 3, n. 1, p. 1-13, 2017. Disponível em: http://repositorio.ipen.br/handle/123456789/28450. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.