Involvent of the NF-kB/p50/Bcl-3 complex in response to antiangiogenic therapy in a mouse model of metastatic renal cell carcinoma
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Biomedicine and Pharmacotherapy
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Renal cell carcinoma (RCC) represents approximately 2–3% of human malignancies. Nuclear
transcription factor kB (NF-kB) is composed of a family of transcription factors that have been
associated with the development and progression of RCC. Endostatin (ES) is a fragment of collagen XVIII
that possesses antiangiogenic activity. In this study, we evaluated the expression of NF-kB in metastatic
tumor cells from animals treated with ES. Balb/c-bearing Renca-EGFP cells were treated with NIH/3T3-
LendSN or NIH/3T3-LXSN cells as a control. At the end of the in vivo experiment, plasma Renca-EGFPsorted cells and tissue lung samples were collected. A real-time PCR array for NF-kB target genes
revealed that ES therapy led to down regulation of Bcl-3 (P < 0.031), NF-kB1 (P < 0.001) and c-Rel
(P < 0.004) in the ES-treated group. Using an electrophoretic mobility shift assay (EMSA), we observed a
reduction in NF-kB binding activity in ES-treated Renca-EGP cells. Furthermore, a supershift assay
showed a clear shift of the NF-kB DNA band in samples incubated with a p50 antibody. By
immunohistochemistry analysis, ES treatment resulted in a significant reduction in expression of p50.
(ES vs. control P < 0.05). The immunoprecipitation experiments confirmed the presence of a p50/Bcl-3
complex in nuclear extracts from cells of metastatic lung tissues. Our findings indicate that p50 and Bcl-3
plays a regulatory role in gene transcription in RCC.
Como referenciar
BRAGA, MARINA de S.; PAIVA, KATIUCIA B. da; FOGUER, KAREN; CHAVES, KAREN C.B.; LIMA, LARISSA de S.; SCAVONE, CRISTOFORO; BELLINI, MARIA H. Involvent of the NF-kB/p50/Bcl-3 complex in response to antiangiogenic therapy in a mouse model of metastatic renal cell carcinoma. Biomedicine and Pharmacotherapy, v. 68, p. 873-879, 2014. DOI: 10.1016/j.biopha.2014.07.008. Disponível em: http://repositorio.ipen.br/handle/123456789/23255. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.