Synthesis and characterization of aminolevulinic acid gold nanoparticles: Photo and sonosensitizer agent for atherosclerosis
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Journal of Luminescence
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Photodynamic and sonodynamic therapies (PDT and SDT, respectively) are emerging as new atherosclerosis
treatments. The subsequent generation of free radicals by activated photo and sonosensitizers can lead to
apoptotic cell death. The use of gold nanoparticles (AuNPs) as the vehicle for a sensitizer delivery improves
reactive oxygen species formation and sensitizer performance. In this study gold nanoparticles functionalized
with polyethylene glycol (PEG) were synthesized mixing δ-aminolevulinic acid (ALA) with tetrachloroauric (III)
acid in milliQ water solution followed by photo reduction with 300W xenon lamp. The synthesized ALA:AuNPs
were characterized by UV/vis optical absorption, zeta potential and electron microscopy. The mean particle size
of spherical ALA:AuNP was ~ 18 nm, with a polydispersity index of 0.437. Singlet oxygen generation efficiency
was measured using the trap molecule 1,3-diphenylisobenzofuran. ALA:AuNPs and DPBF were irradiated with
590 nm LED, or pulse ultrasound irradiation (1 W/cm2 with 1.0 MHz), and consumption of the DPBF was
monitored over time by the absorption and emission spectra. The results showed that he gold nanoparticles
generate singlet oxygen during light and ultrasound irradiations. THP-1 cells differentiated into macrophages
cytotoxicity test were described and was found the half maximal inhibitory concentration (IC50) values ~ 36 nM
for ALA:AuNPs. Increase in the fluorescence intensity of PpIX extracted from macrophages incubated with
ALA:AuNPs indicating stable encapsulation of ALA into gold nanoparticles and further conversion to PpIX. The
potential use of ALA:AuNps as a sensitizer for photo and sonodynamic therapies were investigated. ALA:AuNPs
mediated SDT was more effective than PDT. SDT with ALA:AuNPs induced the reduction of macrophage viability
in ~ 87,5% in only 2 min. The mechanism underlying SDT-induced apoptosis involves the generation of singlet
oxygen. The results indicate that ALA:AuNPs can be used as a novel photo and sonosensitizer for atherosclerosis.
Como referenciar
GONCALVES, KARINA de O.; VIEIRA, DANIEL P.; COURROL, LILIA C. Synthesis and characterization of aminolevulinic acid gold nanoparticles: Photo and sonosensitizer agent for atherosclerosis. Journal of Luminescence, v. 197, p. 317-323, 2018. DOI: 10.1016/j.jlumin.2018.01.057. Disponível em: http://repositorio.ipen.br/handle/123456789/29199. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.