Chemical characterization of bacteria

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2020
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PAN-AMERICAN NANOTECHNOLOGY CONFERENCE, 2nd
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Atomic force microscopy -Infrared spectroscopy (AFM-IR) is a combined technique that allows nanoscale chemical characterization of biological–materials interactions. In this work, AFM-IR was used to map Escherichia coli in graphene oxide /silver nanocomposite (Ag/rGO). In Escherichia coli, it was observed absorption bands corresponding to amine I at 1660 cm-1 and amide II at 1550 cm-1 from proteins. On the other hand, when these bacteria were exposed to (Ag/rGO) typical absorption bands from carbonyl/carboxyl groups around 1745 and carbon bond around 1620 were also detected, showing the antibacterial activities of (Ag/rGO). The conventional atomic force microscope was used to elucidate the morphologic changes that occurred by internalization of nanocomposite into the bacteria. Atomic force microscopy -Infrared spectroscopy (AFM-IR) is a combined technique that allows nanoscale chemical characterization of biological–materials interactions. In this work, AFM-IR was used to map Escherichia coli in graphene oxide /silver nanocomposite (Ag/rGO). In Escherichia coli, it was observed absorption bands corresponding to amine I at 1660 cm-1 and amide II at 1550 cm-1 from proteins. On the other hand, when these bacteria were exposed to (Ag/rGO) typical absorption bands from carbonyl/carboxyl groups around 1745 cm-1 and carbon bond around 1620 cm-1 were also detected, showing the antibacterial activities of (Ag/rGO). The conventional atomic force microscope was used to elucidate the morphologic changes that occurred by internalization of nanocomposite into the bacteria.

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JACOVONE, R.M.; COSTA, C.A.; SAKATA, S.K. Chemical characterization of bacteria: graphene oxide/Ag nanocomposite interactions by atomic force microscopy -infrared spectroscopy. In: PAN-AMERICAN NANOTECHNOLOGY CONFERENCE, 2nd, March 4-7, 2020, Águas de Lindoia, SP. Abstract... Disponível em: http://repositorio.ipen.br/handle/123456789/31874. Acesso em: 16 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.

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