Destabilisation of the SNARE complex by the binding of calcium channel blocking toxin from Phoneutria nigriventer spider venom
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2000
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REUNIAO CIENTIFICA ANUAL DO INTITUTO BUTANTAN
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Aims: This study describes the alterations induced in the neurolransmitter release machinery function by the
interaction of a peptide toxin PF3 which strongly interacts with calcium channels associated to neurotransmitter
release.
Methods and Results: Neurotransmilter release funclion was evaluated by the release of labelled acetylcholine
previously incorporated to the functional pool of neurotransmitter in rat striatal chopped tissue in the presence and
absence of Ca"' using a superfusion apparatus. Results show that in the presence of Ca'', PF3 decreased *HAcetylcholine release while in the absence of Ca" PF3 increased *H-Acetylcoline release. Previous results have
demonstrated that PF3 interacts reversibly with calcium channels in the presence of Ca"' and irreversibly in the
absence of this ion. The strong and irreversible binding of PF3 to the P/Q and N type calcium channel in calcium-free
conditions could destabilise the proteic machinery involved in neurotransmitter release and induce the observed
increase in 3H-acetylcholine release. Alternatively, the lack of Ca** may impair membrane recycling after exocytosis
and consequently, revert the vesicular choline transporter leading to an extrusion of *H-choline producing an
apparent increase in release. These hypotheses will be challenged in future experiments.
Conclusions: These results represent an unexpected effect of the calcium channel blocker PF3, and may represent
a direct proof of the proposed association of calcium channels with the SNARE complex.
Como referenciar
TRONCONE, L.R.P.; ARRUDA PAES, P.C.; CAMILLO, M.A.P. Destabilisation of the SNARE complex by the binding of calcium channel blocking toxin from Phoneutria nigriventer spider venom. In: REUNIAO CIENTIFICA ANUAL DO INTITUTO BUTANTAN, 4-8 dez, 2000, Sao Paulo, SP. Resumos... p. 37. Disponível em: http://repositorio.ipen.br/handle/123456789/21798. Acesso em: 30 Apr 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.