Corncob pretreated with the combination of electron beam irradiation and enzymes to enhance fermentable sugars for biofuel production

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dc.contributor.authorPASCOAL, ERIC S.
dc.contributor.authorKLEINGESINDS, EDUARDO K.
dc.contributor.authorMOURA, ESPERIDIANA A.B.
dc.contributor.authorLUGAO, ADEMAR B.
dc.contributor.authorRODRIGUES, RITA C.L.B.
dc.coverageInternacionalpt_BR
dc.creator.eventoBRAZILIAN BIOENERGY SCIENCE AND TECHNOLOGY CONFERENCEpt_BR
dc.date.accessioned2018-08-16T16:31:37Z
dc.date.available2018-08-16T16:31:37Z
dc.date.eventoOctober 17-19, 2017pt_BR
dc.description.abstractLignocellulosic biomass pretreatment technologies have been taken up as a global challenge as it comprises to increase enzyme accessibility to biomass and yields of fermentable sugars. The reducing sugars released from pretreatment of the corn cob can be converted into biofuels. Corncob is a lignocellulosic material composed of cellulose, hemicellulose, and lignin. Cellulose and hemicellulose are polysaccharides constituted of simple sugars (hexoses and pentoses). However, these sugars are difficult to access, due to the presence of lignin, which is a polyphenolic molecule that provides a high recalcitrance to plant tissue. An appropriate biomass pretreatment disrupts the hydrogen bonds in crystalline cellulose, breaks down cross-linked matrix of hemicelluloses and lignin, and raises the porosity and surface area of cellulose for subsequent enzymatic hydrolysis. There are several pretreatment methods including, physical pretreatment (electron beam irradiation, grinding and milling, microwave, and extrusion), chemical pretreatment (alkali, acid, organosolv, ozonolysis, and ionic liquid), physico-chemical pretreatment (steam explosion, liquid hot water, ammonia fiber explosion, wet oxidation, and CO2 explosion), and biological pretreatment. This study evaluated electron beam irradiation (EB) in combination with enzymatic hydrolysis on corncob at different grain size to produce fermentable sugars. Dry biomass samples after characterization were exposed to EB radiation doses of 0, 30, 50, 70, 100, and 200 kGy. Enzymatic hydrolysis of the pretreated biomass samples were conducted using 10% of solid in Erlenmeyer flasks (125 mL) containing of 50 mL of medium prepared with sodium citrate buffer (50 mmol.L-1, pH 4.8), CellicCTec 2 25.50 FPU/g dry lignocellulosic material) and Tween 80 (9.8 % w/w) under 200 rpm at 50 °C. using the Cellic® CTec2 from Novozymes. The structural changes and degree of crystallinity of the pretreated biomass were studied by FTIR, DRX, DSC, TG and SEM analyses. Corncob in natura showed 6.3 % extractives, 40.3 % cellulose, 31.8 % hemicellulose, 17.3 % lignin, and 0.7 % ash. The highest conversion of cellulose to glucose (44.2%) was by using EB radiation doses of 200 kGy and reduced corn cob grain size. Significant improvement in the enzymatic saccharification (80.4%) of the EBI exposed biomass was observed compared to control. The sugars released can be converted to biofuel or another bioproduct. The EB in combination with enzymatic hydrolysis of corncob is an environmentally sound biomass pretreatment.pt_BR
dc.event.siglaBBESTpt_BR
dc.identifier.citationPASCOAL, ERIC S.; KLEINGESINDS, EDUARDO K.; MOURA, ESPERIDIANA A.B.; LUGAO, ADEMAR B.; RODRIGUES, RITA C.L.B. Corncob pretreated with the combination of electron beam irradiation and enzymes to enhance fermentable sugars for biofuel production. In: BRAZILIAN BIOENERGY SCIENCE AND TECHNOLOGY CONFERENCE, October 17-19, 2017, Campos do Jordão, SP. <b>Abstract...</b> São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular, 2017. Disponível em: http://repositorio.ipen.br/handle/123456789/29063.
dc.identifier.orcidhttps://orcid.org/0000-0002-1737-3191
dc.identifier.orcidhttps://orcid.org/0000-0003-3546-1643
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/29063
dc.localSão Paulo, SPpt_BR
dc.local.eventoCampos do Jordão, SPpt_BR
dc.publisherSociedade Brasileira de Bioquímica e Biologia Molecularpt_BR
dc.rightsopenAccesspt_BR
dc.titleCorncob pretreated with the combination of electron beam irradiation and enzymes to enhance fermentable sugars for biofuel productionpt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorADEMAR BENEVOLO LUGAO
ipen.autorESPERIDIANA AUGUSTA BARRETOS DE MOURA
ipen.codigoautor339
ipen.codigoautor1045
ipen.contributor.ipenauthorADEMAR BENEVOLO LUGAO
ipen.contributor.ipenauthorESPERIDIANA AUGUSTA BARRETOS DE MOURA
ipen.date.recebimento18-08pt_BR
ipen.event.datapadronizada2017pt_BR
ipen.identifier.ipendoc24855pt_BR
ipen.notas.internasAbstractpt_BR
ipen.type.genreResumo
relation.isAuthorOfPublication99ac24c5-2ae1-465a-a6f2-40b4d9af6af7
relation.isAuthorOfPublication6bf804a8-aeed-4e9c-bad7-fdec1b1e9a47
relation.isAuthorOfPublication.latestForDiscovery6bf804a8-aeed-4e9c-bad7-fdec1b1e9a47
sigepi.autor.atividadeMOURA, ESPERIDIANA A.B.:1045:740:Npt_BR
sigepi.autor.atividadeLUGAO, ADEMAR B.:339:740:Npt_BR
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