Optical coherence tomography and polarized light microscopy for the evaluation of artificial caries
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General Dentistry
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This study was designed to investigate whether there is
a correlation between the findings of optical coherence
tomography (OCT) and polarized light microscopy (PLM)
when these techniques are used to evaluate standard
enamel white-spot lesions developed by distinct cariogenic
challenges. Bovine enamel fragments (N = 168)
were randomly allocated into 6 experimental groups
according to the microbiologic model (Streptococcus
mutans UA159, Streptococcus sobrinus ATCC 33478,
or mixed S mutans and S sobrinus) and carbohydrate
sources (1% sucrose or combined 1% sucrose and 1%
starch). Specimens were examined by OCT and PLM
every day within a period of 7 days. Five measurements
of demineralization depth were recorded for each specimen,
and means were calculated. Data were analyzed
with analysis of variance and Tukey tests (α = 0.05), and
a correlation test was performed. All cariogenic challenges
created sub-superficial lesions. In both the OCT
and PLM analyses, the demineralization depth reached
its peak between days 6 and 7 of the cariogenic challenge,
except for the group challenged with S sobrinus
supplemented with combined sucrose and starch; for
that group, demineralization peaked on day 5 in the OCT
analysis. There was a significant correlation between
OCT and PLM (P = 0.00; r = 0.842). This preliminary study
suggests that OCT is a reliable, nondestructive method
to measure the demineralization depth of enamel whitespot
lesions, which can be useful for the laboratory and
has potential for clinical studies. Using the 1% sucrose
and S mutans model for 6 days is a simple and effective
method to induce enamel caries–like lesions without
compromising the depth and morphologic features of
the obtained lesions.
Como referenciar
QUITERO, MAYRA F.Z.; SIRIANI, LUCIANA K.; AZEVEDO, CYNTHIA S. de; FREITAS, ANDERSON Z. de; SCARAMUCCI, TAIS; SIMIONATO, MARIA R.L.; MATOS, ADRIANA B. Optical coherence tomography and polarized light microscopy for the evaluation of artificial caries: a preliminary study. General Dentistry, v. 67, n. 1, p. e1-e6, 2019. Disponível em: http://repositorio.ipen.br/handle/123456789/30858. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.