The irradiation parameters investigation of photodynamic therapy on yeast cells

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dc.contributor.authorPRATES, RENATO A.pt_BR
dc.contributor.authorSILVA, ERIQUES G. dapt_BR
dc.contributor.authorYAMADA JUNIOR, AECIO M.pt_BR
dc.contributor.authorSUZUKI, LUIS C.pt_BR
dc.contributor.authorPAULA, CLAUDETE R.pt_BR
dc.contributor.authorRIBEIRO, MARTHA S.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2014-07-15T13:42:15Zpt_BR
dc.date.accessioned2014-07-30T11:48:15Z
dc.date.available2014-07-15T13:42:15Zpt_BR
dc.date.available2014-07-30T11:48:15Z
dc.date.issued2008pt_BR
dc.description.abstractIt has been proposed that photodynamic therapy (PDT) can inactivate microbial cells. A range of photosensitizers and light sources were reported as well as different fluence parameters and dye concentrations. However, much more knowledge regarding to the role of fluences, irradiation time and irradiance are required for a better understanding of the photodynamic efficiency. The aims of this study were to investigate the role of light parameters on the photoinactivation of yeast cells, and compare cell survivors in different growing phases following PDT. To perform this study, a suspension (106cfu/mL) of Candida albicans ATCC-90028 was used in log and stationary-phase. Three irradiances 100mW/cm2, 200mW/cm2 and 300mW/cm2 were compared under 3min, 6min and 9min of irradiation, resulting in fluences of 18, 36, 54, 72,108 and 162J/cm2. The light source used was a laser emitting at 660nm with output power of 30, 60 and 90mW. As photosensitizer, 100μΜ methylene blue was used. PDT was efficient against yeast cells (6 log reduction) in log and stationary-phase. Neither photosensitizer nor light alone presented any reduction of cell viability. The increase of irradiance and time of irradiation showed a clearly improvement of cell photoinactivation. Interestingly, the same fluences in different irradiances presented dissimilar effects on cell viability. The irradiance and time of irradiation are important in PDT efficiency. Fluence per se is not the best parameter to compare photoinativation effects on yeast cells. The growing-phases presented the same susceptibility under C. albicans photoinactivation.
dc.format.extent6840G1-68460G6pt_BR
dc.identifier.citationPRATES, RENATO A.; SILVA, ERIQUES G. da; YAMADA JUNIOR, AECIO M.; SUZUKI, LUIS C.; PAULA, CLAUDETE R.; RIBEIRO, MARTHA S. The irradiation parameters investigation of photodynamic therapy on yeast cells. <b>Progress in Biomedical Optics and Imaging</b>, v. 6846, p. 6840G1-68460G6, 2008. DOI: <a href="https://dx.doi.org/10.1117/12.763059">10.1117/12.763059</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/4917.
dc.identifier.doi10.1117/12.763059
dc.identifier.issn1605-7422pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/4917pt_BR
dc.identifier.vol6846pt_BR
dc.relation.ispartofProgress in Biomedical Optics and Imagingpt_BR
dc.rightsopenAccessen
dc.subjecttherapypt_BR
dc.subjectlaserspt_BR
dc.subjectmethylene bluept_BR
dc.subjecttissuespt_BR
dc.subjectyeastspt_BR
dc.subjectfungipt_BR
dc.subjectantimicrobial agentspt_BR
dc.subjectphotosensitivitypt_BR
dc.titleThe irradiation parameters investigation of photodynamic therapy on yeast cellspt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorLUIS CLAUDIO SUZUKI
ipen.autorAECIO MASSAYOSHI YAMADA JUNIOR
ipen.autorRENATO ARAÚJO PRATES
ipen.codigoautor574
ipen.codigoautor3953
ipen.codigoautor3248
ipen.codigoautor4028
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorLUIS CLAUDIO SUZUKI
ipen.contributor.ipenauthorAECIO MASSAYOSHI YAMADA JUNIOR
ipen.contributor.ipenauthorRENATO ARAÚJO PRATES
ipen.date.recebimento10-05pt_BR
ipen.identifier.fiSem F.I.pt_BR
ipen.identifier.ipendoc14837pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublication27acc312-4d9c-45d2-bfa7-b51e8f00f5c0
relation.isAuthorOfPublication75bfc40b-e2a3-4593-a784-f7416cfc9837
relation.isAuthorOfPublicationfde20222-8ca2-4a9e-941b-516590cac130
relation.isAuthorOfPublication.latestForDiscoveryfde20222-8ca2-4a9e-941b-516590cac130
sigepi.autor.atividadePRATES, RENATO A.:4028:920:Spt_BR
sigepi.autor.atividadeYAMADA JUNIOR, AECIO M.:3248:-1:Npt_BR
sigepi.autor.atividadeSUZUKI, LUIS C.:3953:920:Npt_BR
sigepi.autor.atividadeRIBEIRO, MARTHA S.:574:920:Npt_BR
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