CTHRSSVVC peptide as a possible early molecular imaging target for atherosclerosis
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International Journal of Molecular Sciences
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The purpose of our work was to select phages displaying peptides capable of binding
to vascular markers present in human atheroma, and validate their capacity to target the vascular
markers in vitro and in low-density lipoprotein receptor knockout (LDLr/) mouse model of
atherosclerosis. By peptide fingerprinting on human atherosclerotic tissues, we selected and isolated
four different peptides sequences, which bind to atherosclerotic lesions and share significant similarity
to known human proteins with prominent roles in atherosclerosis. The CTHRSSVVC-phage peptide
displayed the strongest reactivity with human carotid atherosclerotic lesions (p < 0.05), when
compared to tissues from normal carotid arteries. This peptide sequence shares similarity to a
sequence present in the fifth scavenger receptor cysteine-rich (SRCR) domain of CD163, which
appeared to bind to CD163, and subsequently, was internalized by macrophages. Moreover, the
CTHRSSVVC-phage targets atherosclerotic lesions of a low-density lipoprotein receptor knockout
(LDLr/) mouse model of atherosclerosis in vivo to High-Fat diet group versus Control group. Tetraazacyclododecane-1,4,7,10-tetraacetic acid-CTHRSSVVC peptide (DOTA-CTHRSSVVC) was
synthesized and labeled with 111InCl3 in >95% yield as determined by high performance liquid
chromatography (HPLC), to validate the binding of the peptide in atherosclerotic plaque specimens.
The results supported our hypothesis that CTHRSSVVC peptide has a remarkable sequence for the
development of theranostics approaches in the treatment of atherosclerosis and other diseases.
Como referenciar
SILVA, ROSEMEIRE A.; GIORDANO, RICARDO J.; GUTIERREZ, PAULO S.; ROCHA, VIVIANE Z.; RUDNICKI, MARTINA; KEE, PATRICK; ABDALLA, DULCINEIA S.P.; PUECH-LEAO, PEDRO; CARAMELLI, BRUNO; ARAP, WADIH; PASQUALINI, RENATA; MENEGHETTI, JOSE C.; MARQUES, FABIO L.N.; KHOOBCHANDANI, MENKA; KATTI, KATTESH V.; LUGAO, ADEMAR B.; KALIL, JORGE. CTHRSSVVC peptide as a possible early molecular imaging target for atherosclerosis. International Journal of Molecular Sciences, v. 17, n. 9, p. 1-18, 2016. DOI: 10.3390/ijms17091383. Disponível em: http://repositorio.ipen.br/handle/123456789/27163. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.