Chronic nasal instillation of residual-oil fly ash (ROFA) induced brain lipid peroxidation and behavioral changes in rats
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Inhalation Toxicology
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Several epidemiological studies have linked particulate matter exposure to numerous adverse
health effects on the respiratory, cardiovascular, and reproductive systems (Braga et al., 1999;
Zanobetti et al., 2000; Anderson et al., 2001; Farhat et al., 2005). More recently, ambient levels
of black carbon were associated to impaired cognitive function in children (Suglia et al., 2008),
suggesting that the central nervous system (CNS) may be a target of air pollutants. The present
study was conducted to (a) determine whether chronic residual oil fly ash (ROFA) exposure
promotes behavioral changes and lipid peroxidation in rat brain areas, and (b) determine
whether N-acetylcysteine (NAC), a general antioxidant, prevents these effects. Forty-five-dayold male Wistar rats were exposed or not to ROFA by intranasal instillation and were treated
or not with NAC (150 mg/kg) ip for 30 days. One day later, rats were submitted to the open
field test to evaluate the motor/exploratory activities and emotionality followed by decapitation.
Striatum and cerebellum were dissected to determine lipid peroxidation by the accumulation of
thiobarbituric acid-reactive substances (TBARS). ROFA instillation induced an increase in lipid
peroxidation level in striatum (p = .033) and cerebellum (p = .030), as compared with the control
group. NAC treatment blocked these changes. ROFA promoted a decrease in the frequency of
peripheral walking (p = .006) and a decrease in exploration (p = .001), which were not blocked
by N-acetylcysteine. The present study provides evidence that toxic particles, administered by
the respiratory route, induce oxidative stress in structures of the central nervous system, as well
as behavioral alterations. The administration of NAC reduces lipid peroxidation at the striatum
and cerebellum levels, but does not influence behavioral disturbances.
Como referenciar
ZANCHI, ANA C.; VENTURINI, CARINA D.; SAIKI, MITIKO; SALDIVA, PAULO H.N.; BARROS, HELENA M.T.; RHODEN, CLÁUDIA R. Chronic nasal instillation of residual-oil fly ash (ROFA) induced brain lipid peroxidation and behavioral changes in rats. Inhalation Toxicology, v. 20, n. 9, p. 795-800, 2008. DOI: 10.1080/08958370802009060. Disponível em: http://repositorio.ipen.br/handle/123456789/5023. Acesso em: 30 Dec 2025.
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