Radiosensitization of human prostate cell line lncap by [6]-gingerol
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2017
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ANAIS DA SOCIEDADE BRASILEIRA DE BIOCIENCIAS NUCLEARES
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Introduction: Prostate cancer is the second most prevalent malignancy and second leading cause
of cancer-related deaths among men in the world. Several different diagnostic and therapeutic
approaches have been developed in order to decrease the death rates. A number of experimental and
clinical studies have showed antiproliferative, pro-apoptotic, anti-metastatic and anti-angiogenic
effects of several phytochemicals. [6]-Gingerol (1-[4'-hydroxy-3'-methoxyphenyl]-5-hydroxy-3-
decanone), the major pungent principle of ginger, has anti-oxidant, anti-inflammation and antitumor
promoting activities.
Aim: The purpose of this study was to evaluate the radiosensitizing activity of [6]-Gingerol in the
human prostate cancer cells.
Methods: The viability was assessed (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide) tetrazolium (MTS) assay. The prostate human cells (LNCAP) (2,5×103 cells/well) were
seeded into 96-well plates, after 24 hr they were treated with 150 and 300μg/mL of [6]-Gingerol or
vehicle alone (0.1% DMSO) in serum containing media. After incubation, MTS solution was added
to the plate at a final concentration of 0.5 mg/mL. The cells were incubated for 2 hr in dark at 37.
The resulting MTS-products were determined by measuring the absorbance at 490 nm with ELISA
reader. In the clonogenic cell survival assay, the cells were divided into two groups: A) control, B)
treated with [6]-Gingerol, C) irradiated control and D) treated with [6]-Gingerol and irradiated. The
cells were irradiated by 60Co source in the range from 0 to 15 Gy, using the GammaCell 220 –
Irradiation Unit of Canadian-Atomic Energy Commision Ltd. (CTR-IPEN). After 10-14 days of
culture in normoxia conditions, cell colonies were fixed and stained with methanol 20% and crystal
violet 0.5% and counted. Multiple comparisons were assessed by One-way ANOVA followed by
Bonferroni´s tests with GraphPad Prism version 6.0 software. p< 0.05 was considered statistically
significant.
Results: Our results demonstrated that [6]-Gingerol treatment induced a dose-dependent decrease
in the cell viability. Compared with the vehicle control, the cell viabilities were 75.99 ± 3.56% and
43.06 ± 7.82% when the cells were exposed to 150 μg/mL and 300 μg/mL of [6]-Gingerol,
respectively. Therefore, we observed a significant difference between the treatment groups;
(P<0.01). Then, the effect of [6]-Gingerol (300 μg/mL) on cell radiosensitivity was evaluated. The
clonogenic cell survival assay showed a significant difference between dose-survival curves of
group (A) and (B), (P<0.05) and between the group (C) and (D), (P<0.05). Therefore, [6]-Gingerol
treatment increased the radiosensitivity of LNCaP cells.
Conclusions: The results demonstrated that, besides inducing a dose-dependent apoptosis in
LNCaP human prostate cancer cells, [6]-Gingerol showed a radiosensitizing activity. These findings
suggests it potential as candidate phytochemical agent for combined therapy for prostate cancer.
Como referenciar
SILVA, JOSIAS P.L.; BELLINI, MARIA H. Radiosensitization of human prostate cell line lncap by [6]-gingerol. In: ANAIS DA SOCIEDADE BRASILEIRA DE BIOCIENCIAS NUCLEARES, 09-11 de outubro, 2017, São Paulo, SP. Abstract... Rio de Janeiro, RJ: Sociedade Brasileira de Biociências Nucleares, 2017. p. 115-115. Disponível em: http://repositorio.ipen.br/handle/123456789/28821. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.