Improvement of the methodological strategies to product functionalizes antibodies using Small Angle Neutron Scattering (SANS)
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2021
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CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th
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Antibodies are used by jawed vertebrates for defense against invading pathogens. Usage of those versatile tools in
a plethora of settings in clinics and biomedical sciences hinges on functionalization strategies that retain native
antibody reactivity. To this date, antibody functionalization is performed by trial and error. We aim to reduce costs by
providing general principles to allow the full spectrum of antibody functionalization by correlating functionalized
antibody reactivity to cognate antigen by small angle neutron scattering, SANS, measurements and mathematical
modeling of antibody and antibody-antigen super-complexes, obtained by titration experiments. For this research we
have used for as antibody pure goat anti rabbit immunoglobulin, and for the antigen, pure Horseradish Peroxidase
Preliminary results show that the systems (antibody and antibody-antigen complexes) do not change in the range of
a temperature related to storage temperature (25º C), body temperature (37º C) and 40º C. These results will give
us the pair distribution function of these systems and the results will be viewed in light of published precedence to
highlight areas where future effort is needed to refine such versatile tools and improve their production. However,
between the antibody and the complexes structure, different conformations were observed. The antibody has a
globular structure with a radius of gyration around 33 Å, and the complexes display an elongated cylindrical shape
with radius of gyration around 63 Å. This study shows how the scattering techniques (SANS) can provide useful
information about the conformation of the antibody and antibody-antigen formation and help to shed light in the
understanding the physical, chemical, and structural changes on the organization of these important antibody
functionalization for the immunological system.
Como referenciar
TREMARIN, BEATRIZ; YOKAICHIYA, FABIANO; KELLERMANN, GUINTHER; FRANCO, MARGARETH K.K.D.; STORSBERG, JOACHIM. Improvement of the methodological strategies to product functionalizes antibodies using Small Angle Neutron Scattering (SANS). In: CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th, October 4-8, 2021, São Paulo, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq), 2021. p. 138-138. Disponível em: http://repositorio.ipen.br/handle/123456789/32866. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.