Effect of topical 5-ALA mediated photodynamic therapy on proliferation index of keratinocytes in 4-NQo-induced potentially malignant oral lesions
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Journal of Photochemistry and Photobiology B: Biology
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Fractionation can improve photodynamic therapy (PDT) efficacy for potentially malignant oral lesion
treatment. The aim of this study was to demonstrate the apoptosis/proliferation index of oral keratinocytes after two sessions of topical 5-ALA-mediated PDT in 4-Nitroquinoline-1-oxide-induced potentially
malignant oral lesion, and to suggest the ideal interval between PDT sessions. Immuno-histochemical
tests for proliferating cell nuclear antigen and caspase-3, and terminal deoxynucleotidyl transferase dUTP
nick end labeling (TUNEL) assay were performed at 6 h, 24 h, 48 h, and 72 h time intervals after PDT. The
number of positive cells showing caspase-3 expression was significantly higher, mainly at 6 h after PDT.
In the first cycle of PDT, the highest frequency of positive cells for TUNEL was found at 24 h. At 72 h after
PDT, proliferating cell nuclear antigen positive cells increased significantly, indicating that there was an
epithelial response in direction towards DNA repair and cell proliferation at this time. Because cell proliferation increases and cell death index decreases at 72 h after PDT, it is recommended that the interval
between the PDT sessions must not be longer than 2 days up to total lesion remission.
Como referenciar
BARCESSAT, ANA R.; HUANG, ISAAC; ROSIN, FLAVIA P.; PINTO JUNIOR, DECIO dos S.; ZEZELL, DENISE M. Effect of topical 5-ALA mediated photodynamic therapy on proliferation index of keratinocytes in 4-NQo-induced potentially malignant oral lesions. Journal of Photochemistry and Photobiology B: Biology, v. 126, p. 33-41, 2013. DOI: 10.1016/jjphotobiol.2013.06.011. Disponível em: http://repositorio.ipen.br/handle/123456789/8453. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.